Anti-FOXD1/BF-2抗体(ab129324)

Reviews (1)Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXD1/BF-2 antibody (ab129324)

    Key features and details

    • Goat polyclonal to FOXD1/BF-2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-FOXD1/BF-2抗体

    • 描述

      山羊多克隆抗体to FOXD1/BF-2

    • 宿主

      Goat

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat

    • 免疫原

      Synthetic peptide corresponding to Human FOXD1/BF-2 aa 453-465 (C terminal).
      Sequence:

      SSVENFTARISNC


      Database link: NP_004463.1
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 阳性对照

      • Human Kidney tissue.

    • 常规说明

       This product was previously labelled as FOXD1

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab129324 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    IHC-P Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    靶标

    • 功能

      Transcription factor required for formation of positional identity in the developing retina, regionalization of the optic chiasm and morphogenesis of the kidney. Can neuralize ectodermal cells directly.

    • 序列相似性

      Contains 1 fork-head DNA-binding domain.

    • 细胞定位

      Nucleus.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • Brain Factor 2 antibody
      • FKH L8 antibody
      • FKHL 8 antibody
      • FKHL8 antibody
      • Forkhead (Drosophila) like 8 antibody
      • Forkhead box D1 antibody
      • Forkhead box protein D1 antibody
      • Forkhead drosophila homolog like 8 antibody
      • Forkhead like 8 antibody
      • Forkhead related activator 4 antibody
      • Forkhead related protein FKHL8 antibody
      • Forkhead related transcription factor 4 antibody
      • Forkhead-related protein FKHL8 antibody
      • Forkhead-related transcription factor 4 antibody
      • FOX D1 antibody
      • FOXD 1 antibody
      • foxd1 antibody
      • FOXD1_HUMAN antibody
      • FREAC 4 antibody
      • FREAC-4 antibody
      • FREAC4 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXD1/BF-2 antibody (ab129324)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXD1/BF-2 antibody (ab129324)

      ab129324, at 2.5 µg/ml, staining FOXD1/BF-2 in formalin-fixed, paraffin-embedded Human kidney tissue by Immunohistochemistry.

    文献 (1)

    发表研究结果有使用 ab129324？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab129324 被引用在 1 文献中.

    • Low JH  et al. Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. Cell Stem Cell 25:373-387.e9 (2019). PubMed: 31303547

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXD1 antibody

     Below Average
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formaldehyde
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    Abcam user community

    Verified customer

    提交于 Oct 01 2018

    Question

    Testing discount information to test the ab in ChIP.

    Read More
    Answer

    Thank you very much for your interest in ab129324..

    To our knowledge, ab129324 has not been tested in ChIP. Therefore, I can offer a discount off a future purchase if you buy ab129324 now, test it inChIP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab129324 in ChIP. I will then send a discount code. This code must be issued before purchasing ab129324 so please wait for my reply before ordering.

    2. Purchase ab129324 either by phone, fax, or online (www.abcam.com).

    3. Test it in ChIP.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab129324 turns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com