Anti-FOXD1/BF-2抗体(ab129324)

Reviews (1)Q&A (1)References (1)

Key features and details

  • Goat polyclonal to FOXD1/BF-2
  • Suitable for: ELISA, IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-FOXD1/BF-2抗体
    参阅全部 FOXD1/BF-2 一抗

  • 描述

    山羊多克隆抗体to FOXD1/BF-2

  • 宿主

    Goat

  • 经测试应用

    适用于: ELISA, IHC-Pmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide corresponding to Human FOXD1/BF-2 aa 453-465 (C terminal).
    Sequence:

    SSVENFTARISNC


    Database link: NP_004463.1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • Human Kidney tissue.

  • 常规说明

     This product was previously labelled as FOXD1

     

性能

应用

Our Abpromise guarantee covers the use of ab129324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/16000.
IHC-P Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能

    Transcription factor required for formation of positional identity in the developing retina, regionalization of the optic chiasm and morphogenesis of the kidney. Can neuralize ectodermal cells directly.

  • 序列相似性

    Contains 1 fork-head DNA-binding domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Brain Factor 2 antibody
    • FKH L8 antibody
    • FKHL 8 antibody
    • FKHL8 antibody
    • Forkhead (Drosophila) like 8 antibody
    • Forkhead box D1 antibody
    • Forkhead box protein D1 antibody
    • Forkhead drosophila homolog like 8 antibody
    • Forkhead like 8 antibody
    • Forkhead related activator 4 antibody
    • Forkhead related protein FKHL8 antibody
    • Forkhead related transcription factor 4 antibody
    • Forkhead-related protein FKHL8 antibody
    • Forkhead-related transcription factor 4 antibody
    • FOX D1 antibody
    • FOXD 1 antibody
    • foxd1 antibody
    • FOXD1_HUMAN antibody
    • FREAC 4 antibody
    • FREAC-4 antibody
    • FREAC4 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXD1/BF-2 antibody (ab129324)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXD1/BF-2 antibody (ab129324)

    ab129324, at 2.5 µg/ml, staining FOXD1/BF-2 in formalin-fixed, paraffin-embedded Human kidney tissue by Immunohistochemistry.

文献

发表研究结果有使用 ab129324？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab129324 被引用在 1 文献中.

  • Low JH  et al. Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. Cell Stem Cell 25:373-387.e9 (2019). PubMed: 31303547

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXD1 antibody

 Below Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
Specification
Kidney
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

提交于 Oct 01 2018

Question

Testing discount information to test the ab in ChIP.

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Answer

Thank you very much for your interest in ab129324..

To our knowledge, ab129324 has not been tested in ChIP. Therefore, I can offer a discount off a future purchase if you buy ab129324 now, test it inChIP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab129324 in ChIP. I will then send a discount code. This code must be issued before purchasing ab129324 so please wait for my reply before ordering.

2. Purchase ab129324 either by phone, fax, or online (www.abcam.com).

3. Test it in ChIP.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab129324 turns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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