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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box

Anti-FOXC2抗体(ab5060)

  • Datasheet
Submit a review Q&A (7)References (24)

Product price, shipping and contact information

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Western blot - Anti-FOXC2 antibody (ab5060)
  • Western blot - Anti-FOXC2 antibody (ab5060)
  • Western blot - Anti-FOXC2 antibody (ab5060)

Key features and details

  • Goat polyclonal to FOXC2
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-FOXC2抗体
    参阅全部 FOXC2 一抗
  • 描述

    山羊多克隆抗体to FOXC2
  • 宿主

    Goat
  • 特异性

    The antibody labels FOXC2 in mouse lymphatic primordia, jugular lymph sacs, lymphatic collectors and capillaries. In western blotting the antibody has been tested on FOXC2 overexpressing cell lines but not on endogenous protein.
  • 经测试应用

    适用于: WBmore details
    不适用于: IHC-Fr
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    RHAAPYSYDCTKY

    , corresponding to C terminal amino acids 489-501 of Human FOXC2.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXC
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development
    • Metabolism
    • Types of disease
    • Metabolic disorders

相关产品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

Our Abpromise guarantee covers the use of ab5060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 2 µg/ml. Detects a band of approximately 60-70 kDa (predicted molecular weight: 53 kDa).

A 1 hour primary incubation is recommended for this product.

  • 应用说明
    Is unsuitable for IHC-Fr.
  • 靶标

    • 功能

      Transcriptional activator. Might be involved in the formation of special mesenchymal tissues.
    • 疾病相关

      Defects in FOXC2 are the cause of lymphedema hereditary type 2 (LMPH2) [MIM:153200]; also known as Meige lymphedema. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections, and physical impairment.
      Defects in FOXC2 are a cause of lymphedema-yellow nails (LYYN) [MIM:153300]. LYYN is characterized by yellow, dystrophic, thick and slowly growing nails, associated with lymphedema and respiratory involvement. Lymphedema occurs more often in the lower limbs. It can appear at birth or later in life. Onset generally follows the onset of ungual abnormalities.
      Defects in FOXC2 are a cause of lymphedema-distichiasis (LYD) [MIM:153400]. LYD is characterized by primary limb lymphedema usually starting at puberty (but in some cases later or at birth) and associated with distichiasis (double rows of eyelashes, with extra eyelashes growing from the Meibomian gland orifices).
    • 序列相似性

      Contains 1 fork-head DNA-binding domain.
    • 细胞定位

      Nucleus.
    • Target information above from: UniProt accession Q99958 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 2303 Human
      • Entrez Gene: 14234 Mouse
      • Entrez Gene: 171356 Rat
      • Omim: 602402 Human
      • SwissProt: Q99958 Human
      • SwissProt: Q61850 Mouse
      • SwissProt: Q63246 Rat
      • Unigene: 436448 Human
      • Unigene: 14092 Mouse
      • Unigene: 216723 Rat
      see all
    • 别名

      • Drosphilia Forkhead Homolog Like 14 antibody
      • FKHL 14 antibody
      • FKHL14 antibody
      • Forkhead Box C2 antibody
      • Forkhead box protein C2 antibody
      • Forkhead related protein FKHL14 antibody
      • Forkhead-related protein FKHL14 antibody
      • FOX C2 antibody
      • Foxc2 antibody
      • FOXC2_HUMAN antibody
      • LD antibody
      • Mesenchyme fork head protein 1 antibody
      • Mesenchyme Forkhead 1 antibody
      • MFH 1 antibody
      • MFH 1 protein antibody
      • MFH-1 protein antibody
      • MFH1 antibody
      • Transcription factor FKH 14 antibody
      • Transcription factor FKH-14 antibody
      see all

    图片

    • Western blot - Anti-FOXC2 antibody (ab5060)
      Western blot - Anti-FOXC2 antibody (ab5060)
      Lane 1 : Anti-FOXC2 antibody (ab5060) at 2 µg/ml
      Lane 2 : Anti-FOXC2 antibody (ab5060) at 1 µg/ml

      Lane 1 : nuclear HEK293 lysate in RIPA buffer
      Lane 2 : human pancreas whole cell lysate (negative control) in RIPA buffer

      Lysates/proteins at 35 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 53 kDa
      Observed band size: 60-70 kDa
      why is the actual band size different from the predicted?



      Primary incubation: 1 hour at room temperature.

    • Western blot - Anti-FOXC2 antibody (ab5060)
      Western blot - Anti-FOXC2 antibody (ab5060)
      FoxC2 is highly expressed in COS7 and 293 cells transiently transfected with pcDNA-FOXC2. This figure shows a western blot incubated with the ab5060 antibody after running lysates from COS7 and 293 cells transiently expressing FoxC2 (lane 1 and 3 respectively). Lane 2 shows a negative control of 293 cells transfected with an empty vector. Review by Michal Bell submitted 19 August 2004

      FoxC2 is highly expressed in COS7 and 293 cells transiently transfected with pcDNA-FOXC2. This figure shows a western blot incubated with the ab5060 antibody after running lysates from COS7 and 293 cells transiently expressing FoxC2 (lane 1 and 3 respectively). Lane 2 shows a negative control of 293 cells transfected with an empty vector. Review by Michal Bell submitted 19 August 2004
    • Western blot - Anti-FOXC2 antibody (ab5060)
      Western blot - Anti-FOXC2 antibody (ab5060)
      Anti-FOXC2 antibody (ab5060) at 1 µg/ml + Human breast cancer lysates at 30 µg

      Developed using the ECL technique.

      Predicted band size: 53 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?



      The primary antibody incubated for 1 hour.

    实验方案

    • Recommended IHC-P protocol with ab5060

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (24)

    发表研究结果有使用 ab5060?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5060 被引用在 24 文献中.

    • Norden PR  et al. Shear stimulation of FOXC1 and FOXC2 differentially regulates cytoskeletal activity during lymphatic valve maturation. Elife 9:N/A (2020). PubMed: 32510325
    • Tavian D  et al. FOXC2 Disease Mutations Identified in Lymphedema Distichiasis Patients Impair Transcriptional Activity and Cell Proliferation. Int J Mol Sci 21:N/A (2020). PubMed: 32698337
    • Zhou LJ  et al. Microglia Are Indispensable for Synaptic Plasticity in the Spinal Dorsal Horn and Chronic Pain. Cell Rep 27:3844-3859.e6 (2019). PubMed: 31242418
    • Trost A  et al. Lymphatic markers in the human optic nerve. Exp Eye Res 173:113-120 (2018). IHC ; Human . PubMed: 29746818
    • Munger SJ  et al. Segregated Foxc2, NFATc1 and Connexin expression at normal developing venous valves, and Connexin-specific differences in the valve phenotypes of Cx37, Cx43, and Cx47 knockout mice. Dev Biol 412:173-90 (2016). PubMed: 26953188
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-7 of 7 Abreviews or Q&A

    Question

    Tissues we tried (mouse tissues):
    Venous valve sections
    Lymphatic valve sections
    Fixation:
    Fresh frozen sections (unfixed) and then fixed in acetone for 10 min at -20C.
    Also tried sections fixed in 1% PFA
    Wash buffer 1xPBS plus 0.25% TX100
    Block: 1% Normal Donkey serum in 1xPBS plus 0.25% TX100 for 30 min
    Dilution used for FoxC2 was 1:150
    Time: Tried 2 hrs with primary antibody as well as overnight at 4C
    Secondary antibody: Donkey anti-goat conjugated with Cy3 for 30 minutes
    The staining with fresh frozen sections that were acetone fixed used to work for us but no longer works with this new batch of antibody.
    We are open to all suggestions

    Read More

    Abcam community

    Verified customer

    Asked on Mar 16 2012

    Answer

    Thank you for your reply.

    We do not batch test ab5060 in IHC on frozen sections, we only guarantee it to work in IHC-P, ChIP and western blot. Therefore that would explain why you are not getting similar results, as we do not test for this application.

    Were you seeing weaker signal than before? If so have you tried to increase the primary antibody concentration? Or were you seeing high background?

    Have you tried the antibody in any of the test of the guaranteed applications? If so, does the antibody work in those applications?

    Read More

    Abcam Scientific Support

    回复于 Mar 16 2012

    Question

    We use the FoxC2 antibody from your company, Catology # ab5060. The first batch we received in 2008 worked fine for us. When we were low, we ordered it again. On , we received one vial (lot #GR29681) . We have tried to do immunohistochemistry with it several times since receiving it, but have not been able to get the results we are used to. We are using the same mouse tissues and parameters for staining, so should get similar results.
    Have there been any issues with this lot #? Is there any way to get a fresh aliquot?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 14 2012

    Answer

    Thank you for contacting Abcam.

    The antibody is covered under our Abpromise for six months and is guaranteed to work in WB, ChIP and IHC-P on mouse and human samples. Since you ordered it in July last year, that is outside the six month guarantee but in this case I can make an exception and say that if we cannot resolve the issue you are having with the antibody then I send a replacement antibody.

    To be able to better assist and see if there are any protocol suggestions I can make, can you please send me a copy of the protocol you are using, including species/cell types, primary antibody concentrations and incubations time, any antigen retrieval performed.

    I look forward to your reply and helping you to resolve this issue.

    Read More

    Abcam Scientific Support

    回复于 Mar 14 2012

    Question

    The researcher is using ab5060 to stain human lymphedema sections (formalin fixed paraffin embedded) and has already contacted us for technical help. Dr Spotswood has suggested changing the pH of the antigen retrieval buffer to pH 9 but this has not changed the problem. The researcher has tried both IF and chromogenic detection with an HRP conjugated antibody. Incubation: overnight at 4C 1:200. no permeabilising agent.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 07 2006

    Answer

    I have been able to open the reference Dagenais SL et al. Foxc2 is expressed in developing lymphatic vessels and other tissues associated with lymphedema-distichiasis syndrome. Gene Expr Patterns 4:611-9 (2004) which has used the antibody in immunohistochemistry and I hope the following detailed protocol will help you: "Five micron paraffin sections were de-paraffinized with xylene and then re-hydrated in decreasing ethanol solutions according to standard procedures. Cryosections were incubated 10 min in acetone at ?20 °C and then air dried 15 min. Sections were washed three minutes in PBS before endogenous peroxidase activity was quenched by incubation for 20 min in 1.5% hydrogen peroxide in methanol. After one three-minute wash in PBS, antigen retrieval was performed on paraffin sections. ...For Foxc2... we used high temperature retrieval by incubating 10 min in boiling 0.01 M citrate buffer (pH 6.0), which was followed by cooling for 20 min at room temperature in the acid bath. Sections were washed for 3 min in PBS and then blocked for 10 min with TNB buffer (TSA" Fluorescence Systems) containing 0.1% Triton X-100 (t-Octylphenoxypolyethoxy-ethanol; Sigma, St Louis, MO, USA). Incubation with primary antibodies was 1 h at room temperature in a humidified chamber. Incubations with secondary and tertiary antibodies were 30 min in a humidified chamber. For protein signal amplification, sections were incubated 10 min with Fluorescein Tyramide (TSA" Fluorescence Systems). All incubations were followed by 3× 3-min washes in PBS containing 0.05% Tween 20 (Sigma). After a final series washes, sections were counterstained with DAPI (4?,6-Diamidino-2-phenylindole) in Vectashield mounting medium (Vector Labs, Burlingame, CA, USA). Signals were visualized with a Zeiss Axiophot Fluorescent microscope and imaging was performed using the Olympus DP70 Digital Camera System. Standard protocols were followed for Hematoxylin & Eosin staining." Please do not hesitate to contact us if you still experience problems with this protocol,

    Read More

    Abcam Scientific Support

    回复于 Feb 08 2006

    Question

    Thank you for sending me a new vial of the FoxC2 antibody. I've tried it both with immunofluorescence and western blot and it works well. Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 23 2005

    Answer

    I am delighted to hear you are satisfied with the replacement vial sent following your problem reported on the 3rd March; thank you for taking the time to let me know. Please consider submitting a review and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on Amazon gift vouchers. Thank you again for your kind e-mail,

    Read More

    Abcam Scientific Support

    回复于 Mar 23 2005

    Question

    Customer is using this antibody in IHC-P with mouse gut and mesentary sections. Microwave antigen retrieval as recommended. She used DAB detection. However, saw staining in the cytoplasm as well as the nucleus. Has this been reported before?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 04 2005

    Answer

    Thank you for your phone call. Originally, this product was released as a fast-track antibody and recently we have upgraded it. One of our customers has tested and used it successfully for application in IHC-formalin-fixed paraffin-embedded sections. I don't know if what you are seeing has been reported before. You may want to refer to the following publication, the authors used ab5060 in IHC: Dagenais SL et al. Foxc2 is expressed in developing lymphatic vessels and other tissues associated with lymphedema-distichiasis syndrome. Gene Expr Patterns 4:611-9 (2004). Please let me know if you require further assistance.

    Read More

    Abcam Scientific Support

    回复于 Mar 08 2005

    Question

    In the last year I have been using your antibodies quite a lot and was generally very satisfied. One of the antibodies that I use often is the FoxC2 antibody, ab5060, which so far worked very nicely. Unfortunately I seem to be having problems with the last batch I ordered. I have now run 2 western blots including positive controls which have been tested previously but I see no bands. The lot number of the tube I have now is 86083. As this is a new tube I think there is something wrong with the antibody, therefore I would like to ask you to send me a new antibody, perhaps from a different batch instead of the one I have.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 02 2005

    Answer

    I'm sorry to hear you are having a problem with your recent vial of ab5060. I can certainly offer you a replacement vial if you can provide me with your order details (the Abcam order number of that particular orde and PO number) and I will arrange this as soon as possible. I look forward to hearing from you,

    Read More

    Abcam Scientific Support

    回复于 Mar 03 2005

    Question

    One of our customers uses your FOXC2 antibody AB5060 in IHC. Using a 1/20 dilution and an Alexa 568 labeled secondary antibody, he was able to detect the antigen in IF on cells overexpressing FOXC2 (overexpression is about 10-15x normal mRNA level). Now he tries to detect endogenous FOXC2 in mouse lung and embryos using aceton fixed frozen sections. He has tried dilutions in the range of 1/100 - 1/10 and secondary antibodies labeled with Alexa 488, Alexa 568 and Cy3. He has also tried a sensitive chromogenic detection system (Biotin/HRP), but until now had found no staining at all. So the question is, has Abcam any informations about the use of this antibody for detection of endogenous FOXC2 or could you provide suggestions on how to improve the staining protocol (dilutions, secondary antibodies, positive controls)? Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 16 2004

    Answer

    Thank you for your enquiry. All the information available for this product is listed on the on-line datasheet Originally, this product has been released as a fast-track antibody and recently we have upgraded it. One of our customers has tested and used it successfully for IHC application. It is good to know it works on transfectants but we can't add anything on endogenous staining because we never got any. We would highly appreciate if your customer can send us a review about this product.

    Read More

    Abcam Scientific Support

    回复于 Apr 20 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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