Application
IHC - Wholemount
Sample
Rat Embryo (E13.5 Rat embryo)
Specification
E13.5 Rat embryo
Other product details
Incubation time
99 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS 1x +0.5% Gelatin + 0.5% Triton
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed
Additional data
Additional Notes
Incubated for 7 days at 37°C in primary antibody
2 days in the secondary at 37°C
then cleared using iDisco+ protocol
Imaged with light sheet microscopy
Abcam response
We would like to thank the reviewer for submitting this AbReview. We are sorry to hear that this antibody did not work in your IHC-wholemount experiment using Rat tissue. Based on our internal testing this antibody does not work in IHC-wholemount and as such is not guaranteed by Abcam for use in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alain Chedotal
Verified customer
提交于 Mar 03 2021