Application
IHC - Wholemount
Sample
Mouse Embryo (E8.5 mouse embryo)
Specification
E8.5 mouse embryo
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1xPBS, 2% milk and 0.5% TritonX
Dilution
1/300
Secondary antibody
Dilution
1/750
Name
Non-Abcam antibody was used: Alexa Fluor 647-labeled Donkey Anti-Rabbit IgG Ant
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Additional data
Additional Notes
embryo fixed in 4% PFA o/n at 4¯C, no additional permeabilization step
co-staining with Ecadherin-594 and GFP-488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Heather Wilson
Verified customer
提交于 Aug 15 2017