Anti-FOXA1抗体(ab5089)
Key features and details
- Goat polyclonal to FOXA1
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
重组抗体的批间可重复性更强
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-FOXA1抗体
参阅全部 FOXA1 一抗 -
描述
山羊多克隆抗体to FOXA1 -
宿主
Goat -
经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Pig, Xenopus laevis, Zebrafish
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免疫原
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阳性对照
- 购买相配的WB阳性对照Recombinant Human FOXA1 protein
- WB: HepG2 and MCF7 nuclear cell lysates; Mouse liver lysate. ICC: MCF7 and U2OS cells.
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Ammonium Sulphate Precipitation -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab5089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49.1 kDa). A 1 hour primary incubation at room temperature is recommended for this product. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. |
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| ICC | Use a concentration of 10 µg/ml. |
靶标
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功能
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis. -
组织特异性
Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas. -
序列相似性
Contains 1 fork-head DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3169 Human
- Entrez Gene: 15375 Mouse
- Entrez Gene: 25098 Rat
- Omim: 602294 Human
- SwissProt: P55317 Human
- SwissProt: P35582 Mouse
- SwissProt: P23512 Rat
- Unigene: 163484 Human
see all -
别名
- forkhead box A1 antibody
- Forkhead box protein A1 antibody
- FOX A1 antibody
see all
图片
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Immunocytochemistry - Anti-FOXA1 antibody (ab5089)
Immunofluorescent analysis of paraformaldehyde-fixed, 0.15% TritonX-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling FOXA1 with ab5089 at 10 ug/ml (1h). Image showing nuclear staining in MCF7 cells. An Alexa Fluor 488® secondary antibody was used at 2 ug/ml. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).
Secondary antibody only control: Unimmunized goat IgG (10 ug/ml) followed by an Alexa Fluor 488® secondary antibody at 2ug/ml.
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Western blot - Anti-FOXA1 antibody (ab5089)Lane 1 : Anti-FOXA1 antibody (ab5089) at 0.1 µg/ml
Lane 2 : Anti-FOXA1 antibody (ab5089) at 0.3 µg/ml
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) nuclear cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) nuclear cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 49.1 kDaWestern blot analysis labeling FOXA1 with ab5089.
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Western blot - Anti-FOXA1 antibody (ab5089)Anti-FOXA1 antibody (ab5089) at 0.1 µg/ml + Mouse liver lysate at 35 µg
Predicted band size: 49.1 kDaWestern blot labeling FOXA1 with ab5089 at 0.1 ug/ml.
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Immunocytochemistry - Anti-FOXA1 antibody (ab5089)Immunofluorescent analysis of paraformaldehyde-fixed, 0.15% TritonX-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling FOXA1 with ab5089 at 10 ug/ml (1h). Image showing nuclear staining in U-2 OS cells. An Alexa Fluor 488® secondary antibody was used at 2 ug/ml. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).
Secondary antibody only control: Unimmunized goat IgG (10 ug/ml) followed by an Alexa Fluor 488® secondary antibody at 2ug/ml.
数据表及文件
文献 (66)
ab5089 被引用在 66 文献中.
- Paranjapye A et al. The FOXA1 transcriptional network coordinates key functions of primary human airway epithelial cells. Am J Physiol Lung Cell Mol Physiol N/A:N/A (2020). PubMed: 32432922
- Saravanan B et al. Ligand dependent gene regulation by transient ERa clustered enhancers. PLoS Genet 16:e1008516 (2020). PubMed: 31905229
- Luo Z & Farnham PJ Genome-wide analysis of HOXC4 and HOXC6 regulated genes and binding sites in prostate cancer cells. PLoS One 15:e0228590 (2020). PubMed: 32012197
- Takaku M et al. Cancer-specific mutation of GATA3 disrupts the transcriptional regulatory network governed by Estrogen Receptor alpha, FOXA1 and GATA3. Nucleic Acids Res 48:4756-4768 (2020). PubMed: 32232341
- Cato L et al. ARv7 Represses Tumor-Suppressor Genes in Castration-Resistant Prostate Cancer. Cancer Cell 35:401-413.e6 (2019). PubMed: 30773341
客户评价及客户问答
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