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I was running my experiments with the same reagents comparing the two different FoxA1 antibodies from one day to the next and hence there is no need to troubleshoot. Furthermore, the same protocol and buffers were also used several fold with other antibodies since then without any problems. Hence the low titer in the second delivery from Abcam is just a very low titer. If you do not want to acknowledge that then there is just a waste of time to dwell more on this issue. If you had really wanted to compare the titers then you should have shown a dilution experiment with the two different lots of antibody and a constant amount of antigen. however, the 100-fold difference that we observed should have been evident in almost any kind of experiment along this line. The data you send me are not informative and raises more questions than answers. The only tissue that seem to be from the same extract is the heart (ab29431) when comparing the two different lots but it remains unclear how much sample that was loaded on the two different gels. Furthermore I do not understand have lanes 3 and 7 can appear so different when the ame antibody was used for probing (ab23738) albeit one was chip grade (lanes 5-8). The ChIP grade antibody does not even recognize any band around 50 KDa (= MW of FoxA1) see lane 7. One way to repair these problems, seen from my standpoint, is to give me a corresponding amount of the old lot (ab 23738, lot 459088) if it still remains in stock or alternatively to send my another FoxA1 antibody (e.g. ab55178).If not then I have to buy a FoxA1 antibody from another company and redirect my future orders accordingly.
Asked on Apr 18 2012
Thank you for your response.
As I have stated before, we do take the quality of our products seriously. We no longer have the previous lot in stock. I would be happy to offer a free of charge replacement for this product either of another lot or another product, ab55178. Alternately I may refund the original purchase for you.
Please let me know how you would like to proceed.
回复于 Apr 18 2012