FBJ murine osteosarcoma viral oncogene homolog B antibody
G0/G1 switch regulatory protein 3 antibody
GOS 3 antibody
GOS B antibody
Oncogene FOS B antibody
Oncogene FOSB antibody
Protein fos B antibody
Protein fosB antibody
Western blot - Anti-Fos B antibody [83B1138] (ab11959)
ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB. ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB.
Flow Cytometry - Anti-Fos B antibody [83B1138] (ab11959)
Overlay histogram showing HeLa cells stained with ab11959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11959, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Immunohistochemistry (Frozen sections) - Anti-Fos B antibody [83B1138] (ab11959)Image courtesy of Dr. Karine Thibault, Université Paris Descartes, France
ab11959 Fos B antibody immunostaining (red) in mouse brain cortex showing the striatum. Tissue was fixed with paraformaldehyde, before incubation with the antibody (1/3000) for 18 hours at 25ºC. A donkey anti-mouse Alexa Fluor® 568 conjugated secondary was used at 1/1000.
Zhang L et al. Analgesic Effects of Duloxetine on Formalin-Induced Hyperalgesia and Its Underlying Mechanisms in the CeA. Front Pharmacol9:317 (2018).
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Pirník Z et al. Repeated peripheral administration of lipidized prolactin-releasing peptide analog induces c-fos and FosB expression in neurons of dorsomedial hypothalamic nucleus in male C57 mice. Neurochem Int116:77-84 (2018).
Read more (PubMed: 29601847) »