Anti-Fos B抗体[83B1138] (ab11959)

概述

  • 产品名称
    Anti-Fos B抗体[83B1138]
    参阅全部 Fos B 一抗
  • 描述
    小鼠单克隆抗体[83B1138] to Fos B
  • 宿主
    Mouse
  • 经测试应用
    适用于: Flow Cyt, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    This antibody was raised against a synthetic peptide corresponding to human Fos B.

  • 阳性对照
    • HeLa whole-cell extract.

性能

应用

Our Abpromise guarantee covers the use of ab11959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/500 - 1/5000.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 40.6 kDa).

靶标

图片

  • ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB. ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB.
  • Overlay histogram showing HeLa cells stained with ab11959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11959, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab11959 Fos B antibody immunostaining (red) in mouse brain cortex showing the striatum. Tissue was fixed with paraformaldehyde, before incubation with the antibody (1/3000) for 18 hours at 25ºC. A donkey anti-mouse Alexa Fluor® 568 conjugated secondary was used at 1/1000.

    See Abreview

文献

This product has been referenced in:
  • Zhang L  et al. Analgesic Effects of Duloxetine on Formalin-Induced Hyperalgesia and Its Underlying Mechanisms in the CeA. Front Pharmacol 9:317 (2018). Read more (PubMed: 29692727) »
  • Pirník Z  et al. Repeated peripheral administration of lipidized prolactin-releasing peptide analog induces c-fos and FosB expression in neurons of dorsomedial hypothalamic nucleus in male C57 mice. Neurochem Int 116:77-84 (2018). IHC-P . Read more (PubMed: 29601847) »

See all 9 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain slices)
Permeabilization
Yes - Triton 0.2%
Specification
Brain slices
Blocking step
PBS-Tween 0.05% + Goat Serum 10% + 0.3M Glycine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 21 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - TritonX-100
Specification
Brain
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 15 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Karine Thibault

Verified customer

提交于 Aug 19 2014

Thank you for your enquiry. With this antibody we usually detect a second band of approximately 34 kDa which is delta-FosB. I hope this information will be useful to you. Should you require any other information, please do not hesitate to contact...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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