Key features and details
- Goat polyclonal to Fluorescein (Biotin)
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, In situ hybridization, WB
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
参阅全部 Fluorescein 一抗
描述山羊多克隆抗体to Fluorescein (Biotin)
经测试应用适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, In situ hybridization, WBmore details
种属反应性与反应: Species independent
Fluorescein conjugated to Goat IgG
Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Our Abpromise guarantee covers the use of ab6655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|ELISA||1/10000 - 1/50000.|
|IHC-P||1/1000 - 1/5000.|
|IHC-Fr||1/1000 - 1/5000.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|In situ hybridization||Use at an assay dependent concentration.|
|WB||1/2000 - 1/10000.|
相关性Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
Anti-Fluorescein antibody (Biotin) (ab6655) at 1/1000 dilution + BSA conjugated Fluorescein.
Peroxidase conjugated Streptavidin secondary antibody at 1/40000 dilution
Immunohistochemical analysis of chicken embryo neuroepithelium labelling fluorescein-tagged morpholinos with ab6655. Samples were fixed in 4% paraformaldehyde at 4°C for 1 hour.
ab6655 被引用在 2 文献中.
- He B et al. Vascular targeting of LIGHT normalizes blood vessels in primary brain cancer and induces intratumoural high endothelial venules. J Pathol 245:209-221 (2018). PubMed: 29603739
- Long K et al. Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin. Nat Commun 7:10354 (2016). IHC-Fr, IHC-FrFl . PubMed: 26838601