Anti-Fluorescein抗体(ab6644)

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Western blot - Anti-Fluorescein antibody (ab6644)

    Key features and details

    • Goat polyclonal to Fluorescein
    • Suitable for: ELISA, Flow Cyt, IHC-Fr, WB
    • Reacts with: Species independent
    • Isotype: IgG

    概述

    • 产品名称

      Anti-Fluorescein抗体
      参阅全部 Fluorescein 一抗

    • 描述

      山羊多克隆抗体to Fluorescein

    • 宿主

      Goat

    • 经测试应用

      适用于: ELISA, Flow Cyt, IHC-Fr, WBmore details

    • 种属反应性

      与反应: Species independent

    • 免疫原

      Fluorescein conjugated to Goat IgG

    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 纯化说明

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab6644 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    ELISA 1/5000 - 1/25000.
    Flow Cyt Use at an assay dependent concentration.
    IHC-Fr Use at an assay dependent concentration.
    WB 1/500 - 1/3000.

    靶标

    • 相关性

      Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.

    • 别名

      • FI antibody
      • FITC antibody
      • fluorescein isothyocianate antibody
      • fluorescyl antibody

    图片

    • Western blot - Anti-Fluorescein antibody (ab6644)
      Western blot - Anti-Fluorescein antibody (ab6644)
      Anti-Fluorescein antibody (ab6644) at 1/1000 dilution + BSA conjugated Fluorescein at 0.05 µg

      Secondary
      Goat secondary antibody (HRP) at 1/40000 dilution

      Observed band size: 67 kDa
      why is the actual band size different from the predicted?



      Primary antibody incubated overnight at 4°C.
      Secondary antibody incubated for 30 minutes at room temperature.

    文献 (0)

    发表研究结果有使用 ab6644？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6644 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    Today I have spoken on the phone to Danielle Miller about your Goat anti-fluoresceine AB6644. We do not have signal with the antibody, only an aspecific band. I have filled in a form via your website about the problems we have. Attached to this mail are two files with images of the blots we obtain: First:' blotabcam' which contains 3 samples and 2 markers. On the image should be clear that nr 1 is the negative control and 2 and 3 are the positive controls.This blot is detected with your antibody (1:1000)and as secondary antibody we have used donkey anti goat with peroxidase(1:5000) from Jackson Immuno Research. Second: ' blotbiogenisis.jpg' which contains 3 samples and 2 markers. On the image should be clear that nr 1 is the negative control and 2 and 3 are the positive controls.This blot is detected with rabbit anti fluoresceine conjugated with a peroxidase. The lot nr of our antibody is 8001. We would like to obtain another lot of the antibody to test again.

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    Answer

    Thank you for you e-mail and providing more details of your protocol. We are going to send you a new vial from a different batch (13597) - free of charge. For your information, the new order number is 42198. Please do let us know how you are gettin on this vial. Good luck!

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