Fluo-8 AM,green fluorescent calcium binding dye (ab142773)
Key features and details
- Medium affinity green fluorescent calcium binding dye. Cell-permeable.
- CAS Number: 1345980-40-6
Soluble in DMSO
- Form / State: Solid
- Source: Synthetic
概述
-
产品名称
Fluo-8 AM,green fluorescent calcium binding dye -
描述
Medium affinity green fluorescent calcium binding dye. Cell-permeable. -
常规说明
Fluo-8® AM Working Solution: On the day of the experiment, either dissolve Fluo-8® AM in DMSO or thaw an aliquot of the indicator stock solution to room temperature. Prepare a dye working solution of 2 to 20 µM in a buffer of your choice (e.g., Hanks and Hepes buffer) with 0.04% Pluronic® F-127. For most cell lines, Fluo-8® AM at a final concentration of 4-5 μM is recommended. The exact concentration of indicators required for cell loading must be determined empirically.
-
CAS编号
1345980-40-6 -
化学结构
性能
-
激发
490nm -
发射
520nm -
化学名称
Bis(acetoxymethyl) 2,2'-((4-(6-(acetoxymethoxy)-3-oxo-3H-xanthen-9-yl)-2-(2-(bis(2-acetoxymethoxy)-2-oxoethyl)amino)phenoxy)ethoxy)phenyl)azanediyl)diacetate
-
分子量
1046.94 -
分子式
C50H50N2O23 -
存放说明
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO
-
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
For more information on AM esters please visit our AM esters FAQ page.Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
SMILES
CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c5ccccc5OCCOc1cc(ccc1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C=2c4ccc(cc4OC3=CC(=O)C=CC=23)OCOC(C)=O -
来源
Synthetic
-
研究领域
图片
-
2D chemical structure image of ab142773, Fluo-8 AM, green fluorescent calcium binding dye
-
FFA treatments increase Ca2+ influx to induce ERS in β-TC3 cells.
β-TC3 cells were incubated with FFA or BSA for 16 h, and then stimulated with 4 µM thapsigargin for 20 min to activate store-operated Ca2+ entry. Fluorescence densities of Ca2+ change were monitored in Fluo-8/AM-loaded β-TC3 cells after FFA or BSA treatments.
(From Figure 2B of Cui et al)
-
U2OS cells were seeded overnight at 40,000 cells per 100 µL per well in a 96-well black wall/clear bottom costar plate. The growth medium was removed, and the cells were incubated with 100 µl of 4 µM Fluo-4 AM or Fluo-8® AM in HHBS at 37 °C, 5% CO2 incubator for 1 hour. The cells were washed twice with 200 µl HHBS, then imaged with a fluorescence microscope using FITC channel.
-
Fluo-8 vs Fluo-4 sensitivity to calcium release in HEK-293 cells induced by Carbachol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (49)
ab142773 被引用在 49 文献中.
- Chechekhin V et al. Alpha1A- and Beta3-Adrenoceptors Interplay in Adipose Multipotent Mesenchymal Stromal Cells: A Novel Mechanism of Obesity-Driven Hypertension. Cells 12:N/A (2023). PubMed: 36831252
- Wang M et al. Axin2 coupled excessive Wnt-glycolysis signaling mediates social defect in autism spectrum disorders. EMBO Mol Med 15:e17101 (2023). PubMed: 37078424
- You W et al. Stressed neuronal cells can recover from profound membrane blebbing, nuclear condensation and mitochondrial fragmentation, but not from cytochrome c release. Sci Rep 13:11045 (2023). PubMed: 37422517
- Ji L et al. Secretogranin III Selectively Promotes Vascular Leakage in the Deep Vascular Plexus of Diabetic Retinopathy. Int J Mol Sci 24:N/A (2023). PubMed: 37445707
- Zhang L et al. MAP Kinase FgHog1 and Importin β FgNmd5 Regulate Calcium Homeostasis in Fusarium graminearum. J Fungi (Basel) 9:N/A (2023). PubMed: 37504696