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Neuroscience Neurotransmission Secretory Vesicles Other

Anti-Flotillin 2/ESA抗体(ab96507)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (5)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (ab96507)
  • Western blot - Anti-Flotillin 2/ESA antibody (ab96507)
  • Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 2/ESA antibody (ab96507)
  • Immunoprecipitation - Anti-Flotillin 2/ESA antibody (ab96507)
  • Western blot - Anti-Flotillin 2/ESA antibody (ab96507)
  • Immunoprecipitation - Anti-Flotillin 2/ESA antibody (ab96507)
  • Western blot - Anti-Flotillin 2/ESA antibody (ab96507)

Key features and details

  • Rabbit polyclonal to Flotillin 2/ESA
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Flotillin 2/ESA抗体
    参阅全部 Flotillin 2/ESA 一抗
  • 描述

    兔多克隆抗体to Flotillin 2/ESA
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IHC-P, ICC/IF, IPmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Flotillin 2/ESA aa 365-428 (C terminal).
    Database link: Q14254

  • 阳性对照

    • WB: HeLa. Mouse brain lysate. Rat brain lysate. IP: Human HeLa whole cell lysate. IHC-P: human lung cancer. ICC/IF: HeLa S3 cells.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Other
    • Signal Transduction
    • Adapters
    • Transmembrane
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Flotillin 2/ESA protein (ab131919)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab96507于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P
1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining.
ICC/IF
1/100 - 1/1000.
IP (1)
1/100 - 1/500.
说明
WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P
1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining.
ICC/IF
1/100 - 1/1000.
IP
1/100 - 1/500.

靶标

  • 功能

    May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
  • 组织特异性

    In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
  • 序列相似性

    Belongs to the band 7/mec-2 family. Flotillin subfamily.
  • 细胞定位

    Cell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae.
  • Target information above from: UniProt accession Q14254 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 2319 Human
    • Entrez Gene: 14252 Mouse
    • Entrez Gene: 83764 Rat
    • Omim: 131560 Human
    • SwissProt: Q14254 Human
    • SwissProt: Q60634 Mouse
    • SwissProt: Q9Z2S9 Rat
    • Unigene: 514038 Human
    • Unigene: 130227 Mouse
    • Unigene: 2105 Rat
    see all
  • 别名

    • ECS-1 antibody
    • ECS1 antibody
    • Epidermal surface antigen 1 antibody
    • Epidermal surface antigen antibody
    • ESA antibody
    • ESA1 antibody
    • Flot2 antibody
    • FLOT2_HUMAN antibody
    • Flotillin 2 (epidermal surface antigen 1) antibody
    • Flotillin-2 antibody
    • M17S1 antibody
    • Membrane component chromosome 17 surface marker 1 antibody
    • Membrane component chromosome 17 surface marker 1 homolog antibody
    • membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1) antibody
    • Membrane component, chromosome 17, surface marker 1 antibody
    • REG-1 antibody
    • Reggie-1 antibody
    • Reggie-2 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (ab96507)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (ab96507)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using ab96507 at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

  • Western blot - Anti-Flotillin 2/ESA antibody (ab96507)
    Western blot - Anti-Flotillin 2/ESA antibody (ab96507)
    Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 2/ESA antibody (ab96507)
    Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 2/ESA antibody (ab96507)
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa S3 cells, using ab96507 at 1:100 dilution. Image 2: Merged with DNA probe.
  • Immunoprecipitation - Anti-Flotillin 2/ESA antibody (ab96507)
    Immunoprecipitation - Anti-Flotillin 2/ESA antibody (ab96507)

    Flotillin 2 was immunoprecipitated from 5µ of HeLa whole cell lysate with ab96507. Western blot was performed from the immunoprecipitate using ab96507. EasyBlot anti-Rabbit IgG, was used as secondary antibody.


    Lane 1: HeLa lysate 5µg (Input).
    Lane 2: ab96507 IP in HeLa lysate.


  • Western blot - Anti-Flotillin 2/ESA antibody (ab96507)
    Western blot - Anti-Flotillin 2/ESA antibody (ab96507)
    Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + Rat brain lysate at 50 µg

    Predicted band size: 47 kDa



    10% SDS-PAGE

  • Immunoprecipitation - Anti-Flotillin 2/ESA antibody (ab96507)
    Immunoprecipitation - Anti-Flotillin 2/ESA antibody (ab96507)This image is courtesy of an Abreview submitted by Prof Ritva Tikkanen

    Flotillin 2/ESA immunoprecipitation from Human HeLa whole cell lysate using ab96507. 750 µg of cell lysate was incubated with primary antibody (1/500) and matrix (Protein A) and incubated for 16 hours at 4°C.

    Lane 1 : anti-Flotillin 2/ESA IP (using non-Abcam antibody)
    Lane 2 : anti-Flotillin 2/ESA IP (using ab96507)
    Lane 3 : Input (HeLa cell lysate)
    Lane 4 : Supernatant IP (using non-Abcam antibody)
    Lane 5 : Supernatant IP (using ab96507)

    Western blot detection : mouse anti-Flotillin 2/ESA antibody + HRP donkey anti-mouse polyclonal secondary antibody

    See Abreview

  • Western blot - Anti-Flotillin 2/ESA antibody (ab96507)
    Western blot - Anti-Flotillin 2/ESA antibody (ab96507)
    Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg

    Predicted band size: 47 kDa



    10% SDS PAGE

实验方案

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (5)

发表研究结果有使用 ab96507?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab96507 被引用在 5 文献中.

  • Bai C  et al. Cryopreservation disrupts lipid rafts and heat shock proteins in yellow catfish sperm. Cryobiology 87:32-39 (2019). PubMed: 30876909
  • Li T  et al. FLOT2 overexpression is associated with the progression and prognosis of human colorectal cancer. Oncol Lett 17:2802-2808 (2019). PubMed: 30854055
  • Gong J  et al. Exosomes mediate cell contact-independent ephrin-Eph signaling during axon guidance. J Cell Biol 214:35-44 (2016). PubMed: 27354374
  • Zhu Q  et al. Overexpression of acylglycerol kinase is associated with poorer prognosis and lymph node metastasis in nasopharyngeal carcinoma. Tumour Biol 37:3349-57 (2016). WB, IHC . PubMed: 26443540
  • Wang X  et al. Flotillin-2 is associated with breast cancer progression and poor survival outcomes. J Transl Med 11:190 (2013). WB . PubMed: 23945257

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Immunoprecipitation abreview for Anti-Flotillin 2/ESA antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
750 µg
Immuno-precipitation step
Protein A
Specification
HeLa
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

PROF. Ritva Tikkanen

Verified customer

提交于 Jan 11 2013

Question

Dear Abcam team,

my group is working on the flotillin family of proteins. I have just gone through the antibodies that you offer for these proteins and found two interesting polyclonal ones.

Flotillin-1 ab41927

Flotillin-2 ab96507

These antibodies have been tested for several applications. However, we would like to test these antibodies in immunoprecipitation, which is so far not included in your list. Would the testing discount option be available for this purpose? These antibodies appear to be multipurpose ones, so that I am sure that many customers would be even more interested to buy them if IP was included. Furthermore, I think that we would pretty fast be able to add a reference publication for these Abs.

Looking forward to your reply! Sincerely,

Read More

Abcam community

Verified customer

Asked on Sep 13 2012

Answer

I am very pleased to hear you would like to test both ab41927 and ab96507 in immunoprecipitation. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for immunoprecipitation and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

回复于 Sep 13 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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