Anti-FKBP52抗体[Hi52C] (ab59460)
Key features and details
- Mouse monoclonal [Hi52C] to FKBP52
- Suitable for: Flow Cyt, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2a
概述
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产品名称
Anti-FKBP52抗体[Hi52C]
参阅全部 FKBP52 一抗 -
描述
小鼠单克隆抗体[Hi52C] to FKBP52 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to human FKBP52
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阳性对照
- IHC-P: Human prostate tissue; Mouse backskin tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
Hi52C -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59460于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. -
组织特异性
Widely expressed. -
序列相似性
Contains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats. -
结构域
The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400. -
翻译后修饰
Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 2288 Human
- Entrez Gene: 14228 Mouse
- Omim: 600611 Human
- SwissProt: Q02790 Human
- SwissProt: P30416 Mouse
- Unigene: 524183 Human
- Unigene: 713721 Human
- Unigene: 12758 Mouse
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别名
- 51 kDa FK506-binding protein antibody
- 52 kDa FK506 binding protein antibody
- 52 kDa FK506-binding protein antibody
see all
图片
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ICC/IF image of ab59460 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59460, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Paraffin-embedded mouse backskin tissue stained for FKBP52 using ab59460 at 1/100 dilution in immunohistochemical analysis.
Fixation: Bouin's Fixative. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 dilution for 1 hour at room temperature.
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ab59460 at 1/100 dilution staining FKBP52 in prostate tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP conjugated goat anti mouse secondary was used at 1/10 dilution.
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Overlay histogram showing HeLa cells stained with ab59460 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab59460, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab59460 被引用在 3 文献中.
- Hong Y et al. Amelioration of muscle wasting by glucagon-like peptide-1 receptor agonist in muscle atrophy. J Cachexia Sarcopenia Muscle N/A:N/A (2019). PubMed: 31020810
- Harrell CS et al. Pharmacological stimulation of hypoxia inducible factor-1a facilitates the corticosterone response to a mild acute stressor. Neurosci Lett 600:75-9 (2015). PubMed: 26037418
- Pare JM et al. Hsp90 cochaperones p23 and FKBP4 physically interact with hAgo2 and activate RNA interference-mediated silencing in mammalian cells. Mol Biol Cell 24:2303-10 (2013). PubMed: 23741051