Anti-FKBP52抗体(ab2926)

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Western blot - Anti-FKBP52 antibody (ab2926)
  • Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody (ab2926)

Key features and details

  • Rabbit polyclonal to FKBP52
  • Suitable for: ICC/IF, WB
  • Reacts with: Rat, Human, African green monkey
  • Isotype: IgG

概述

  • 产品名称

    Anti-FKBP52抗体
    参阅全部 FKBP52 一抗

  • 描述

    兔多克隆抗体to FKBP52

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WBmore details

  • 种属反应性

    与反应: Rat, Human, African green monkey

  • 免疫原

    Synthetic peptide corresponding to Rabbit FKBP52 aa 441-458.
    Sequence:

    KDERNDVAGSQSQVETEA

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab2926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB 1/1000. Detects a band of approximately 52 kDa.

靶标

  • 功能

    Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.

  • 组织特异性

    Widely expressed.

  • 序列相似性

    Contains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.

  • 结构域

    The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
    The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
    The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.

  • 翻译后修饰

    Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 细胞定位

    Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 51 kDa FK506-binding protein antibody
      • 52 kDa FK506 binding protein antibody
      • 52 kDa FK506-binding protein antibody
      • 52 kDa FKBP antibody
      • 59 kDa immunophilin antibody
      • FK506 binding protein 4 antibody
      • FK506-binding protein 4 antibody
      • FKBP 4 antibody
      • FKBP 52 antibody
      • FKBP 59 antibody
      • FKBP-4 antibody
      • FKBP-52 antibody
      • FKBP4 antibody
      • FKBP4_HUMAN antibody
      • FKBP51 antibody
      • FKBP52 protein antibody
      • FKBP59 antibody
      • HBI antibody
      • Hsp 56 antibody
      • HSP binding immunophilin antibody
      • HSP-binding immunophilin antibody
      • Hsp56 antibody
      • Immunophilin FKBP52 antibody
      • N-terminally processed antibody
      • p52 antibody
      • p59 antibody
      • p59 protein antibody
      • Peptidyl prolyl cis trans isomerase antibody
      • peptidyl-prolyl cis-trans isomerase antibody
      • Peptidyl-prolyl cis-trans isomerase FKBP4 antibody
      • Peptidylprolyl cis trans isomerase antibody
      • PPIase antibody
      • PPIase FKBP4 antibody
      • Rotamase antibody
      • T cell FK506 binding protein 59kD antibody
      see all

    图片

    • Western blot - Anti-FKBP52 antibody (ab2926)
      Western blot - Anti-FKBP52 antibody (ab2926)
      All lanes : Anti-FKBP52 antibody (ab2926) at 1/1000 dilution

      Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
      Lane 4 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
      Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 6 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
      Lane 7 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : goat anti-rabbit IgG-HRP secondary antibody at 1/20000 dilution


      Western blot analysis of FKBP52 was performed by loading samples onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was incubated with ab2926 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a secondary antibody for at least 1 hour. Chemiluminescent detection was performed.

    • Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody (ab2926)
      Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody (ab2926)

      Immunocytochemistry/Immunofluorescence analysis of FKBP52 (green) in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) ab2926 (1:100) for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (1:400) for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

    文献 (1)

    发表研究结果有使用 ab2926？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2926 被引用在 1 文献中.

    • Ardi VC  et al. Macrocycles that inhibit the binding between heat shock protein 90 and TPR-containing proteins. ACS Chem Biol 6:1357-66 (2011). PubMed: 21950602

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