Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antibody Diluent (Dako)
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
45 minute(s) · Temperature: 20°C · Diluent: Antibody Diluent (Dako)
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit HRP polymer (pre-diluted, Dako)
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Would recommend diluting even more than 1/2000 to reduce background.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
提交于 Apr 02 2009