Fix & Perm / Cell Fixation & Permeabilization试剂盒(Flow Cytometry) (ab185917)

概述

  • 产品名称

    Fix & Perm / Cell Fixation & Permeabilization试剂盒(Flow Cytometry)
  • 产品概述

    Fix & Perm / Cell Fixation and Permeabilization Kit ab185917 is used to improve the staining of intracellular markers for flow cytometry. It is used to fix cells in suspension and then to permeabilize the cell membranes. It can be used for both cytoplasmic, mitochondrial and nuclear markers, as well as markers in other cellular organelles.


    The reagents allow antibodies access to intracellular structures and leave the morphological scatter characteristics of cells intact. The formulation reduces background staining and allows the simultaneous addition of the permeabilization medium and fluorochrome labeled antibodies.

  • 说明

    The kit protocol is very simple:

    - incubate cells with Fixation Medium for 15 min

    - add PBS and spin down cells

    - add Permeabilization Medium and antibody to cell pellet

    - incubate for 15 min

    - wash cells with PBS and analyze

    The overwhelming majority of flow cytometry antibodies are suitable for use with this kit (a limited number are sensitive to the fixation step).

  • 经测试应用

    适用于: Flow Cytmore details

性能

应用

Our Abpromise guarantee covers the use of ab185917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

图片

  • Flow cytometric analysis of normal bone marrow (BM) after fixation and pemeabilization using Fix and Perm kit ab185917, followed by immunostaining for the intra-cellular antigens Lactoferrin and MPO.

实验方案

文献

ab185917 has not yet been referenced specifically in any publications.

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