FITC荧光Anti-S100A9 + Calprotectin (S100A8/A9 complex)抗体[MAC387] (ab7429)

Reviews (1)Q&A (3)References (7)
Flow Cytometry (Intracellular) - FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab7429)

    Key features and details

    • FITC Mouse monoclonal [MAC387] to S100A9 + Calprotectin (S100A8/A9 complex)
    • Suitable for: Flow Cyt (Intra)
    • Reacts with: Human, Monkey
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Carrier Free Unconjugated

    概述

    • 产品名称

      FITC荧光Anti-S100A9 + Calprotectin (S100A8/A9 complex)抗体[MAC387]
      参阅全部 S100A9 + Calprotectin (S100A8/A9 complex) 一抗

    • 描述

      FITC荧光小鼠单克隆抗体[MAC387] to S100A9 + Calprotectin (S100A8/A9 complex)

    • 宿主

      Mouse

    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    • 特异性

      This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.

    • 经测试应用

      适用于: Flow Cyt (Intra)more details

    • 种属反应性

      与反应: Human, Monkey

    • 免疫原

      Human monocytes

    • 阳性对照

      • Flow Cyt (Intra): Human peripheral blood granulocyte

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab7429于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt (Intra)
    Use 10µl for 106 cells.

    Membrane permeabilisation is required for this application. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    说明
    Flow Cyt (Intra)
    Use 10µl for 106 cells.

    Membrane permeabilisation is required for this application. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    图片

    • Flow Cytometry (Intracellular) - FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab7429)
      Flow Cytometry (Intracellular) - FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab7429)

      ab7429 staining S100A9 + Calprotectin (FITC) in human peripheral blood granulocyte by Flow Cytometry analysis.

    文献 (7)

    发表研究结果有使用 ab7429？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab7429 被引用在 7 文献中.

    • Monteiro LN  et al. Tumour-associated macrophages: Relation with progression and invasiveness, and assessment of M1/M2 macrophages in canine mammary tumours. Vet J 234:119-125 (2018). IHC-P . PubMed: 29680383
    • Burwitz BJ  et al. Technical advance: liposomal alendronate depletes monocytes and macrophages in the nonhuman primate model of human disease. J Leukoc Biol 96:491-501 (2014). PubMed: 24823811
    • Gutierrez M  et al. The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet Immunol Immunopathol 71:321-34 (1999). PubMed: 10587310
    • Brandtzaeg P  et al. The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function. Histopathology 21:191-6 (1992). PubMed: 1505941
    • Brandtzaeg P  et al. Mac 387 antibody and detection of formalin resistant myelomonocytic L1 antigen. J Clin Pathol 41:963-70 (1988). PubMed: 3056988
    • Flavell DJ  et al. Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J Histochem Cytochem 35:1217-26 (1987). PubMed: 3309045
    • Taraballi F  et al. Biomimetic collagenous scaffold to tune inflammation by targeting macrophages. J Tissue Eng 7:2041731415624667 (0). IHC . PubMed: 26977285

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    1-4 of 4 Abreviews or Q&A

    Flow Cytometry abreview for Anti-Macrophage antibody [MAC387] (FITC)

     Average
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Rat Cell (macrophages (peritoneal))
    Specification
    macrophages (peritoneal)
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    Mr. olivier Reynard

    Verified customer

    提交于 Jul 24 2006

    Question

    This antibody seems to cross react with granulocytes and monocytes as well. How is the specificity for macrophages determined for this antibody?

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    Answer

    Thank you for contacting us.

    This antibody recognized the L1 or Calprotectin molecule which is expressed by granulocytes, monocytes and tissue macrophages. In staining samples by flow cytometry which contains all of these relevant populations, I would expect positive staining in all groups.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
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    Question

    I am looking for an antibody to identify macrophage from monocyte by western blot. We have your product CD14 (cat#ab14006-50), but it seems positive for both monocyte and macrophage. Is there any other product we could use (for human cells)?

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    Answer

    After a careful search with the sources of our macrophage antibodies I regret to inform you that we have no products in our product range which can identify macrophage form monocyte by Western blot and are cross reactive with human. In our product range we have ab8173 monoclonal antibody specific for rat macrophage. Unfortunately this antibody is not cross reactive with human. My apologies for the inconvenience,

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    Question

    Is it possible to use the above fluorescently labelled antibody for immunohistochemistry on frozen tissue sections?

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    Answer

    This antibody has not yet been tested in frozen sections.

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