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Tags & Cell Markers Epitope Tags Myc Tag

FITC荧光Anti-Myc tag抗体(ab1394)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)

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Key features and details

  • FITC Chicken polyclonal to Myc tag
  • Suitable for: WB, Flow Cyt
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgY

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概述

  • 产品名称

    FITC荧光Anti-Myc tag抗体
    参阅全部 Myc tag 一抗
  • 描述

    FITC荧光鸡多克隆抗体to Myc tag
  • 宿主

    Chicken
  • 偶联物

    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用

    适用于: WB, Flow Cytmore details
  • 免疫原

    EQKLISEEDL ( C-MYC) conjugated with KLH.

  • 常规说明

    Molar F/P ratio is 5.6:1

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • 纯度

    IgY fraction
  • 纯化说明

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • 克隆

    多克隆
  • 同种型

    IgY
  • 轻链类型

    unknown
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • Myc Tag

相关产品

  • Immunizing Peptide (Blocking)

    • Human c-Myc peptide (ab13837)

应用

Our Abpromise guarantee covers the use of ab1394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Can be blocked with Human c-Myc peptide (ab13837).
Flow Cyt 1/100.

靶标

  • 相关性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • 细胞定位

    Nuclear
  • 别名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

实验方案

  • Western blot protocols
  • Flow cytometry protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (0)

    发表研究结果有使用 ab1394?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1394 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Question

    Abcam technical, I have ab63498 (chicken (IgY) polyclonal anti-Human Serum Albumin antibody (FITC)) and I am looking to purchase a suitable negative chicken IgY control. Could you suggest one from your category that preferably does not cross react with a human antigen? For example, perhaps antibody ab1394 a chicken polyclonal antibody to c-myc or ab63504, a chicken polyclonal to bacteria Protein L (FITC). I am using the anti-albumin antibody in a flow cytometry application. Thanks!

    Read More

    Abcam community

    Verified customer

    Asked on Jan 06 2012

    Answer

    Thank you for contacting us. Unfortunately, I would not recommend using a chicken antibody against a different target (such as ab1394 or ab63504), as it cannot be guaranteed that they do not react with any human antigen. I would rather suggest to use a chicken IgY polyclonal isotype control, as these isotype controls are specifically tested not to cross react. As such we currently have ab37382 available (Click here (or use the following: https://www.abcam.com/Chicken-IgY-polyclonal-Isotype-Control-BSA-and-Azide-free-ab37382.html).). As this antibody however is not labelled with FITC, I may recommend our EasyLink Conjugation kit (ab102883 - ab102885, see below and attached). For the compensation and set-up of the flow cytometer, I would suggest to use ab63498 to ensure the optimal settings. EasyLink antibody conjugation kits: https://www.abcam.com/easylink Click here (or use the following: https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-1-x-1mg-FITC-ab102883.html). Having said this however, I may let you know that isotype controls do not really represent a good negative control. The reasons become apparent in your particular case: The control antibody is of a different preparation ('chicken', or in case of monoclonals a different clone) and therefore may show varying binding properties compared to the specific antibody of interest (ab63498). Furthermore, as the conjugation conditions may have been different, the labelling with the fluorochrome can vary which can be a very important issue in flow cytometry. As a result of different antibody-to-fluorochrome ratios and/or different fluorochrome batches, the control antibody might indeed show a very different staining pattern, and even if it does not bind at all (as it is supposed to) can for these reasons not directly be compared to the antibody of interest. Instead, in flow cytometry another type of control is often used to determine positivity for the marker of interest. This control is termed fluorescence minus one or FMO and works like an isotype control without the actual antibody. FMO controls are set up by leaving out one of the antibodies in your staining panel. You may find more information about this type of control in the latest publications or on flow cytometry websites, such as http://www.flowcytometryuk.org/, http://www.cyto.purdue.edu/ or http://www.isac-net.org . Plus, your local flow cytometry community (core facility?) might be happy to help and advise in the use of optimal controls which are required for publishing the results. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Jan 06 2012

    Flow Cytometry abreview for Anti-Myc tag antibody (FITC)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Saccharomyces cerevisiae Cell (Yeast expressing myc-tagged peptide)
    Specification
    Yeast expressing myc-tagged peptide
    Fixation
    none
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    提交于 May 21 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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