FITC荧光Anti-Integrin alpha 4/CD49D抗体[44H6] (ab221)
![Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [44H6] (ab221)](https://www.abcam.cn/ps/products/0/ab221/Images/ab221-3436-anti-integrin-alpha-4-antibody-44h6-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [44H6] (ab221)")
Key features and details
- FITC Mouse monoclonal [44H6] to Integrin alpha 4/CD49D
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
FITC荧光Anti-Integrin alpha 4/CD49D抗体[44H6]
参阅全部 Integrin alpha 4/CD49D 一抗 -
描述
FITC荧光小鼠单克隆抗体[44H6] to Integrin alpha 4/CD49D -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
44H6 recognises the alpha chain of VLA 4. It reacts with ALL and AML cells, T-cells, B-cells and monocytes. No reactivity is observed in human kidney or brain sections. 44H6 will induce homotypic aggregation. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Integrin alpha 4/CD49D. HOON pre-B leukaemia cell line
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常规说明
Purified IgG conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.6 : 1.0.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant -
克隆
单克隆 -
克隆编号
44H6 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 10µl for 106 cells.
or 100µl whole blood.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
| 说明 |
|---|
|
Flow Cyt
Use 10µl for 106 cells. or 100µl whole blood.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
靶标
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功能
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
结构域
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion. -
翻译后修饰
Phosphorylation on Ser-1027 inhibits PXN binding. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3676 Human
- Omim: 192975 Human
- SwissProt: P13612 Human
- Unigene: 440955 Human
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别名
- 269C wild type antibody
- Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
- CD49 antigen like family member D antibody
see all
图片
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Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [44H6] (ab221)
ab221 staining Integrin alpha 4/CD49D (FITC) in human peripheral blood lymphocytes by Flow Cytometry analysis.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab221 被引用在 2 文献中.
- Huang S et al. BMP2 Is Related to Hirschsprung's Disease and Required for Enteric Nervous System Development. Front Cell Neurosci 13:523 (2019). PubMed: 31849612
- Chen X et al. RANKL signaling in bone marrow mesenchymal stem cells negatively regulates osteoblastic bone formation. Bone Res 6:34 (2018). PubMed: 30510839