FITC荧光Anti-Integrin alpha 4/CD49D抗体[44H6] (ab221)

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Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [44H6] (ab221)

    Key features and details

    • FITC Mouse monoclonal [44H6] to Integrin alpha 4/CD49D
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    概述

    • 产品名称

      FITC荧光Anti-Integrin alpha 4/CD49D抗体[44H6]
      参阅全部 Integrin alpha 4/CD49D 一抗

    • 描述

      FITC荧光小鼠单克隆抗体[44H6] to Integrin alpha 4/CD49D

    • 宿主

      Mouse

    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    • 特异性

      44H6 recognises the alpha chain of VLA 4. It reacts with ALL and AML cells, T-cells, B-cells and monocytes. No reactivity is observed in human kidney or brain sections. 44H6 will induce homotypic aggregation.

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      HOON pre-B leukaemia cell line

    • 常规说明

      Purified IgG conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.6 : 1.0.

       This product was previously labelled as Integrin alpha 4

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab221于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use 10µl for 106 cells.

    or 100µl whole blood.

     

     

     

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     
    说明
    Flow Cyt
    Use 10µl for 106 cells.

    or 100µl whole blood.

     

     

     

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    靶标

    • 功能

      Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

    • 序列相似性

      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.

    • 结构域

      The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.

    • 翻译后修饰

      Phosphorylation on Ser-1027 inhibits PXN binding.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • 269C wild type antibody
        • Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
        • CD49 antigen like family member D antibody
        • CD49 antigen-like family member D antibody
        • CD49d antibody
        • IA4 antibody
        • Integrin alpha 4 antibody
        • Integrin alpha 4 subunit antibody
        • Integrin alpha IV antibody
        • Integrin alpha-4 antibody
        • Integrin alpha-IV antibody
        • Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody
        • ITA4_HUMAN antibody
        • ITGA4 antibody
        • MGC90518 antibody
        • OTTHUMP00000205320 antibody
        • Very late activation protein 4 receptor, alpha 4 subunit antibody
        • VLA 4 subunit alpha antibody
        • VLA-4 subunit alpha antibody
        • VLA4 antibody
        see all

      图片

      • Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [44H6] (ab221)
        Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [44H6] (ab221)

        ab221 staining Integrin alpha 4/CD49D (FITC) in human peripheral blood lymphocytes by Flow Cytometry analysis.

      文献 (2)

      发表研究结果有使用 ab221？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab221 被引用在 2 文献中.

      • Huang S  et al. BMP2 Is Related to Hirschsprung's Disease and Required for Enteric Nervous System Development. Front Cell Neurosci 13:523 (2019). PubMed: 31849612
      • Chen X  et al. RANKL signaling in bone marrow mesenchymal stem cells negatively regulates osteoblastic bone formation. Bone Res 6:34 (2018). PubMed: 30510839

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