FITC荧光Anti-Integrin alpha 4/CD49D抗体[44H6] (ab221)
Key features and details
- FITC Mouse monoclonal [44H6] to Integrin alpha 4/CD49D
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
概述
-
产品名称
FITC荧光Anti-Integrin alpha 4/CD49D抗体[44H6]
参阅全部 Integrin alpha 4/CD49D 一抗 -
描述
FITC荧光小鼠单克隆抗体[44H6] to Integrin alpha 4/CD49D -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
44H6 recognises the alpha chain of VLA 4. It reacts with ALL and AML cells, T-cells, B-cells and monocytes. No reactivity is observed in human kidney or brain sections. 44H6 will induce homotypic aggregation. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
HOON pre-B leukaemia cell line
-
常规说明
Purified IgG conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.6 : 1.0.
This product was previously labelled as Integrin alpha 4
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
-
纯度
Protein A purified -
纯化说明
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant -
克隆
单克隆 -
克隆编号
44H6 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt | Use 10µl for 106 cells. or 100µl whole blood.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
靶标
-
功能
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
结构域
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion. -
翻译后修饰
Phosphorylation on Ser-1027 inhibits PXN binding. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 3676 Human
- Omim: 192975 Human
- SwissProt: P13612 Human
- Unigene: 440955 Human
-
别名
- 269C wild type antibody
- Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
- CD49 antigen like family member D antibody
see all
图片
文献 (2)
ab221 被引用在 2 文献中.
- Huang S et al. BMP2 Is Related to Hirschsprung's Disease and Required for Enteric Nervous System Development. Front Cell Neurosci 13:523 (2019). PubMed: 31849612
- Chen X et al. RANKL signaling in bone marrow mesenchymal stem cells negatively regulates osteoblastic bone formation. Bone Res 6:34 (2018). PubMed: 30510839