FITC荧光Anti-Integrin alpha 4/CD49D抗体[44H6] (ab221)

Submit a review Submit a question References (2)
Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [44H6] (ab221)

    Key features and details

    • FITC Mouse monoclonal [44H6] to Integrin alpha 4/CD49D
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    概述

    • 产品名称

      FITC荧光Anti-Integrin alpha 4/CD49D抗体[44H6]
      参阅全部 Integrin alpha 4/CD49D 一抗

    • 描述

      FITC荧光小鼠单克隆抗体[44H6] to Integrin alpha 4/CD49D

    • 宿主

      Mouse

    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    • 特异性

      44H6 recognises the alpha chain of VLA 4. It reacts with ALL and AML cells, T-cells, B-cells and monocytes. No reactivity is observed in human kidney or brain sections. 44H6 will induce homotypic aggregation.

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      HOON pre-B leukaemia cell line

    • 常规说明

      Purified IgG conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.6 : 1.0.

       This product was previously labelled as Integrin alpha 4

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab221 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Flow Cyt Use 10µl for 106 cells.

    or 100µl whole blood.

     

     

     

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    靶标

    • 功能

      Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

    • 序列相似性

      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.

    • 结构域

      The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.

    • 翻译后修饰

      Phosphorylation on Ser-1027 inhibits PXN binding.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • 269C wild type antibody
        • Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
        • CD49 antigen like family member D antibody
        • CD49 antigen-like family member D antibody
        • CD49d antibody
        • IA4 antibody
        • Integrin alpha 4 antibody
        • Integrin alpha 4 subunit antibody
        • Integrin alpha IV antibody
        • Integrin alpha-4 antibody
        • Integrin alpha-IV antibody
        • Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody
        • ITA4_HUMAN antibody
        • ITGA4 antibody
        • MGC90518 antibody
        • OTTHUMP00000205320 antibody
        • Very late activation protein 4 receptor, alpha 4 subunit antibody
        • VLA 4 subunit alpha antibody
        • VLA-4 subunit alpha antibody
        • VLA4 antibody
        see all

      图片

      • Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [44H6] (ab221)
        Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [44H6] (ab221)

        ab221 staining Integrin alpha 4/CD49D (FITC) in human peripheral blood lymphocytes by Flow Cytometry analysis.

      文献 (2)

      发表研究结果有使用 ab221？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab221 被引用在 2 文献中.

      • Huang S  et al. BMP2 Is Related to Hirschsprung's Disease and Required for Enteric Nervous System Development. Front Cell Neurosci 13:523 (2019). PubMed: 31849612
      • Chen X  et al. RANKL signaling in bone marrow mesenchymal stem cells negatively regulates osteoblastic bone formation. Bone Res 6:34 (2018). PubMed: 30510839

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      There are currently no Customer reviews or Questions for ab221.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com