FITC荧光Anti-CD90 / Thy1抗体[MRC OX-7] (ab226)
Key features and details
- FITC Mouse monoclonal [MRC OX-7] to CD90 / Thy1
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Rat
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
概述
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产品名称
FITC荧光Anti-CD90 / Thy1抗体[MRC OX-7]
参阅全部 CD90 / Thy1 一抗 -
描述
FITC荧光小鼠单克隆抗体[MRC OX-7] to CD90 / Thy1 -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
Recognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c. -
Tested Applications & Species
Application Species Flow Cyt RatICC/IF Rat -
免疫原
corresponding to CD90/ Thy1.
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阳性对照
- Flow Cyt: PC12 cells. ICC/IF: PC12 cells
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常规说明
The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.
Conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.0 : 1.0.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant -
克隆
单克隆 -
克隆编号
MRC OX-7 -
骨髓瘤
NS1 -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Alternative Versions
- HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219)
- PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab216354)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (ab222781)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
- PE Anti-CD90 / Thy1 antibody [MRC OX-7] (ab33694)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
---|---|
Flow Cyt |
Rat
|
ICC/IF |
Rat
|
All applications |
Mouse
Rabbit
Guinea pig
|
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
1/500.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
|
ICC/IF |
1/100.
This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min) |
说明 |
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Flow Cyt
1/500. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
ICC/IF
1/100. This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min) |
靶标
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功能
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 21838 Mouse
- Entrez Gene: 24832 Rat
- SwissProt: P01831 Mouse
- SwissProt: P01830 Rat
- Unigene: 3951 Mouse
- Unigene: 108198 Rat
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别名
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
图片
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ab226 staining CD90 / Thy1 in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab226 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Overlay histogram showing PC12 cells stained with ab226 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab226, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Mouse IgG1 (monoclonal) FITC (ab18435) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab226 被引用在 16 文献中.
- Tarafder S et al. Engineered Healing of Avascular Meniscus Tears by Stem Cell Recruitment. Sci Rep 8:8150 (2018). PubMed: 29802356
- Pak S et al. Endoscopic Transplantation of Mesenchymal Stem Cell Sheets in Experimental Colitis in Rats. Sci Rep 8:11314 (2018). PubMed: 30054522
- Li J et al. Carbon monoxide releasing molecule-3 promotes the osteogenic differentiation of rat bone marrow mesenchymal stem cells by releasing carbon monoxide. Int J Mol Med 41:2297-2305 (2018). PubMed: 29393384
- Chopra H et al. Lapine periodontal ligament stem cells for musculoskeletal research in preclinical animal trials. J Transl Med 16:174 (2018). PubMed: 29929550
- Sun AJ et al. Comparison of mouse brown and white adipose-derived stem cell differentiation into pacemaker-like cells induced by TBX18 transduction. Mol Med Rep 17:7055-7064 (2018). PubMed: 29568953