Key features and details
- FITC Rabbit polyclonal to C3c
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
参阅全部 C3c 一抗
偶联物FITC. Ex: 493nm, Em: 528nm
特异性This antibody reacts with human C3c complement and with the C3c part of C3 and C3b.
经测试应用适用于: ICC/IFmore details
预测可用于: Mouse, Rat, Sheep, Goat, Guinea pig, Cow, Cat, Dog, Pig, Kangaroo, Mink
Full length native protein (purified) corresponding to Human C3c. C3c complement isolated from complement activated human serum.
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存放说明Shipped at 4°C. Store at +4°C.
存储溶液Preservative: 0.05% Sodium azide
Constituent: 1% BSA
Concentration information loading...
纯化说明Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins.
The Abpromise guarantee
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml.
功能C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
疾病相关Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
序列相似性Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain.
翻译后修饰C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
Phosphorylation sites are present in the extracelllular medium.
- Information by UniProt
形式Cleaved into the following 10 chains: 1) Complement C3 beta chain 2) Complement C3 alpha chain 3) C3a anaphylatoxin 4) Complement C3b alpha' chain 5) Complement C3c alpha' chain fragment 1 6) Complement C3dg fragment 7) Complement C3g fragment 8) Complement C3d fragment 9) Complement C3f fragment 10) Complement C3c alpha' chain fragment 2
- acylation-stimulating protein cleavage product antibody
- AHUS5 antibody
- ARMD9 antibody
ICC/IF image of ab4212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4212, 1µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab4212 被引用在 7 文献中.
- Pan T et al. Circulating Heme Oxygenase-1 and Complement Activation in Transplant-Associated Thrombotic Microangiopathy. Biol Blood Marrow Transplant N/A:N/A (2019). PubMed: 30871975
- Li Y et al. Antidepressant-Like Action of Single Facial Injection of Botulinum Neurotoxin A is Associated with Augmented 5-HT Levels and BDNF/ERK/CREB Pathways in Mouse Brain. Neurosci Bull N/A:N/A (2019). PubMed: 30900142
- Ispasanie E et al. Spontaneous point mutations in the capsule synthesis locus leading to structural and functional changes of the capsule in serogroup A meningococcal populations. Virulence 9:1138-1149 (2018). PubMed: 30067453
- Noone DG et al. Von Willebrand factor regulates complement on endothelial cells. Kidney Int 90:123-34 (2016). PubMed: 27236750
- Bahia El Idrissi N et al. Complement activation at the motor end-plates in amyotrophic lateral sclerosis. J Neuroinflammation 13:72 (2016). PubMed: 27056040
- Zhang Y et al. Effect of the glycosyltransferases on the capsular polysaccharide synthesis of Streptococcus suis serotype 2. Microbiol Res 185:45-54 (2016). PubMed: 26946377
- Kim SJ et al. Intravitreal human complement factor H in a rat model of laser-induced choroidal neovascularisation. Br J Ophthalmol 97:367-70 (2013). Rat . PubMed: 23258212