FITC荧光Anti-Biotin抗体(ab6650)

Submit a review Q&A (2)References (2)

Key features and details

  • FITC Goat polyclonal to Biotin
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

概述

  • 产品名称

    FITC荧光Anti-Biotin抗体
    参阅全部 Biotin 一抗

  • 描述

    FITC荧光山羊多克隆抗体to生物素

  • 宿主

    Goat

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

  • 经测试应用

    适用于: Flow Cyt, ICC/IFmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Chemical/ Small Molecule conjugated to KLH

  • 常规说明

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

相关产品

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6650于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use at an assay dependent concentration.

ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/500 - 1/2500.
说明
Flow Cyt
Use at an assay dependent concentration.

ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/500 - 1/2500.

靶标

  • 相关性

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

  • 别名

    • Vitamin B4 antibody

文献 (2)

发表研究结果有使用 ab6650？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6650 被引用在 2 文献中.

  • Link J  et al. Transient and Partial Nuclear Lamina Disruption Promotes Chromosome Movement in Early Meiotic Prophase. Dev Cell 45:212-225.e7 (2018). PubMed: 29689196
  • Baratta JL  et al. Cellular organization of normal mouse liver: a histological, quantitative immunocytochemical, and fine structural analysis. Histochem Cell Biol 131:713-26 (2009). IHC-FoFr . PubMed: 19255771

客户评价及客户问答

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Question

Hello, Can you tell me how many dyes (fluorescein) are attached per molecule of anti-biotin antibody? Waiting for your reply and thanking you in advance. Cheers.

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Answer

The molar ratios of dye-to-protein are varied batch to batch. The general acceptance criteria for this product is 2-5:1

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Question

Have you used AB6650 (Goat anti-biotin Fluorescein conjugate) for membrane-based detection of Biotinylated nucleic acids on charged nylon membranes (specifically Gene-screen plus)? If so, what membrane blocking reagents have been tested & how did they work? (i.e best, 2nd best etc.)

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Answer

To our knowledge this antibody has yet to be tested in this application. We would welcome any feedback in this respect from researchers who have tried using this product.

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