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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin

FITC荧光Anti-beta Actin抗体[AC-15] (ab6277)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (17)

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Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)
  • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)
  • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)

Key features and details

  • FITC Mouse monoclonal [AC-15] to beta Actin
  • Suitable for: ICC/IF
  • Reacts with: Rat, Chicken
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

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概述

  • 产品名称

    FITC荧光Anti-beta Actin抗体[AC-15]
    参阅全部 beta Actin 一抗
  • 描述

    FITC荧光小鼠单克隆抗体[AC-15] to beta Actin
  • 宿主

    Mouse
  • 偶联物

    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用

    适用于: ICC/IFmore details
  • 种属反应性

    与反应: Rat, Chicken
  • 免疫原

    Synthetic peptide:

    DDDIAALVIDNGSGL

    conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 表位

    The N-terminal of the beta isoform of actin
  • 阳性对照

    • Cultured human or chicken fibroblasts. Molecular weight of beta actin is 42Kd.
  • 常规说明

    Immunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque.


    Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 纯度

    IgG fraction
  • 纯化说明

    Purified Immunoglobulin
  • Primary antibody说明

    Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells
  • 克隆

    单克隆
  • 克隆编号

    AC-15
  • 同种型

    IgG1
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

相关产品

  • Alternative Versions

    • HRP Anti-beta Actin antibody [AC-15] (ab49900)
    • Anti-beta Actin antibody [AC-15] (ab6276)
  • Isotype control

    • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
  • Recombinant Protein

    • Recombinant Human beta Actin protein (His tag) (ab240844)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6277于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (1)
Use at an assay dependent concentration.
说明
ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 疾病相关

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • 序列相似性

    Belongs to the actin family.
  • 翻译后修饰

    ISGylated.
  • 细胞定位

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 396526 Chicken
    • Entrez Gene: 81822 Rat
    • SwissProt: P60706 Chicken
    • SwissProt: P60711 Rat
    • Unigene: 94978 Rat
    • 别名

      • A26C1A antibody
      • A26C1B antibody
      • ACTB antibody
      • ACTB_HUMAN antibody
      • Actin beta antibody
      • Actin cytoplasmic 1 antibody
      • Actin, cytoplasmic 1, N-terminally processed antibody
      • Actx antibody
      • b actin antibody
      • b-actin antibody
      • Beta cytoskeletal actin antibody
      • Beta-actin antibody
      • BRWS1 antibody
      • E430023M04Rik antibody
      • Melanoma X actin antibody
      • MGC128179 antibody
      • PS1TP5 binding protein 1 antibody
      • PS1TP5BP1 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)

      ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence. 

      30 min blocking with TBS-T / 5% BSA
      1 hr incubation with ab6277 (TBS-T / 5% BSA)
      1 minute nuclear counterstaining with DAPI

      Magnification x20

    • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)

      Immunocytochemistry/ Immunofluorescence analysis of chicken fibroblast cells labeling beta Actin with ab6277 at 1/300 dilution. The cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 1/300 Anti-beta Actin antibody [AC-15] (FITC) (ab6277).

       

    • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)

      ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.

      30 min blocking with TBS-T / 5% BSA
      1 hr incubation with ab6277 (TBS-T / 5% BSA)
      1 minute nuclear counterstaining with DAPI

      Magnification x40 no deconvolution.

    实验方案

    • Direct IF

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (17)

    发表研究结果有使用 ab6277?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6277 被引用在 17 文献中.

    • Cagnetta R  et al. Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring. Mol Cell 73:474-489.e5 (2019). PubMed: 30595434
    • Réu P  et al. A 61% lighter cell culture dish to reduce plastic waste. PLoS One 14:e0216251 (2019). PubMed: 31039189
    • Cagnetta R  et al. Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome. Neuron 99:29-46.e4 (2018). PubMed: 30008298
    • Olthoff JT  et al. Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle. Nat Commun 9:5104 (2018). PubMed: 30504831
    • Llewellyn KJ  et al. A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy. PLoS One 10:e0131995 (2015). WB . PubMed: 26134519
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Actin antibody [AC-15] (FITC)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Lung)
    Permeabilization
    Yes - 0.1% Triton X
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. James Xiao

    Verified customer

    提交于 Oct 31 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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