FITC荧光Anti-6X His tag®抗体(ab3554)

Reviews (1)Q&A (1)References (1)

Key features and details

  • FITC Chicken polyclonal to 6X His tag®
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgY

概述

  • 产品名称

    FITC荧光Anti-6X His tag®抗体
    参阅全部 6X His tag® 一抗

  • 描述

    FITC荧光鸡多克隆抗体to 6X His tag®

  • 宿主

    Chicken

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

  • 经测试应用

    适用于: WB, ELISAmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Synthetic peptide: HHHHHH conjugated to KLH.

  • 常规说明

    Molar F/P ratio is 3.6:1.

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 7.00
    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 骨髓瘤

    unknown

  • 同种型

    IgY

  • 轻链类型

    unknown

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3554于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration.

Prior to conjugation, the antibodies were analyzed by Western Blot.
ELISA
Use at an assay dependent concentration.

Prior to conjugation, the antibodies were analyzed by ELISA.
说明
WB
Use at an assay dependent concentration.

Prior to conjugation, the antibodies were analyzed by Western Blot.
ELISA
Use at an assay dependent concentration.

Prior to conjugation, the antibodies were analyzed by ELISA.

靶标

  • 相关性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

  • 细胞定位

    Depends upon the localization of the parent protein tagged with hexahistidine.

  • 别名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

文献 (1)

发表研究结果有使用 ab3554？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab3554 被引用在 1 文献中.

  • Del Toro D  et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell 180:323-339.e19 (2020). PubMed: 31928845

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-6X His tag® antibody (FITC)

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 21°C
Sample
Rat Tissue sections (spinal cord tissue)
Specification
spinal cord tissue
Permeabilization
Yes - TritonX
Fixative
Paraformaldehyde
Read More

Dr. Hayk Petrosyan

Verified customer

提交于 Jun 23 2014

Question

How do you see the westeren blot results with FITC conugated Ab? Thanks

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Answer

I cannot advise performing western blotting with a FITC antibody although it is likely to work given a suitable secondary antibody that recognizes the primary chicken antiserum. The reason behind the fact that western blotting is a listed application is that the antibody is derived from a parent antibody (the same as ab3553, an HRP conjugated 6X His tag antibody) and has been shown from the HRP form to work by western blotting. I would however recommend ab3554 for use by western blotting and ab3553 for western blotting.

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