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Tags & Cell Markers Epitope Tags His Tag

FITC荧光Anti-6X His tag®抗体(ab3554)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (1)

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Key features and details

  • FITC Chicken polyclonal to 6X His tag®
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgY

概述

  • 产品名称

    FITC荧光Anti-6X His tag®抗体
    参阅全部 6X His tag® 一抗
  • 描述

    FITC荧光鸡多克隆抗体to 6X His tag®
  • 宿主

    Chicken
  • 偶联物

    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用

    适用于: WB, ELISAmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Synthetic peptide: HHHHHH conjugated to KLH.

  • 常规说明

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Molar F/P ratio is 3.6:1.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 7.00
    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 骨髓瘤

    unknown
  • 同种型

    IgY
  • 轻链类型

    unknown
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • His Tag

应用

Our Abpromise guarantee covers the use of ab3554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.

Prior to conjugation, the antibodies were analyzed by Western Blot.

ELISA Use at an assay dependent concentration.

Prior to conjugation, the antibodies were analyzed by ELISA.

靶标

  • 相关性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

实验方案

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (1)

    发表研究结果有使用 ab3554?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3554 被引用在 1 文献中.

    • Del Toro D  et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell 180:323-339.e19 (2020). PubMed: 31928845

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-6X His tag® antibody (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 21°C
    Sample
    Rat Tissue sections (spinal cord tissue)
    Specification
    spinal cord tissue
    Permeabilization
    Yes - TritonX
    Fixative
    Paraformaldehyde
    Read More

    Dr. Hayk Petrosyan

    Verified customer

    提交于 Jun 23 2014

    Question

    How do you see the westeren blot results with FITC conugated Ab? Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Sep 22 2005

    Answer

    I cannot advise performing western blotting with a FITC antibody although it is likely to work given a suitable secondary antibody that recognizes the primary chicken antiserum. The reason behind the fact that western blotting is a listed application is that the antibody is derived from a parent antibody (the same as ab3553, an HRP conjugated 6X His tag antibody) and has been shown from the HRP form to work by western blotting. I would however recommend ab3554 for use by western blotting and ab3553 for western blotting.

    Read More

    Abcam Scientific Support

    回复于 Sep 23 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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