FITC荧光Anti-6X His tag®抗体(ab1270)

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Key features and details

  • FITC Goat polyclonal to 6X His tag®
  • Suitable for: ICC
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

概述

  • 产品名称

    FITC荧光Anti-6X His tag®抗体
    参阅全部 6X His tag® 一抗

  • 描述

    FITC荧光山羊多克隆抗体to 6X His tag®

  • 宿主

    Goat

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

  • 经测试应用

    适用于: ICCmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Synthetic peptide:

    HHHHHH

    (6-His) conjugated to KLH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab1270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/200 - 1/2000.

靶标

  • 相关性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

  • 细胞定位

    Depends upon the localization of the parent protein tagged with hexahistidine.

  • 别名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

文献 (1)

发表研究结果有使用 ab1270？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab1270 被引用在 1 文献中.

  • Tai-Nagara I  et al. Placental labyrinth formation in mice requires endothelial FLRT2/UNC5B signaling. Development 144:2392-2401 (2017). PubMed: 28576770

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Customer woul like to know: Does ab1270 react with the N terminus or C terminus end of 6-His?

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Answer

The antibody is designed to react with 6 His at the N-terminus and C-terminus.

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Question

I was wondering about the fixatives used for this antibody. Is paraformaldehyde ok, or must I use methanol, etc. More importantly, can this antibody be used on unfixed cells to detect the presence of a cell-surface HIS-tagged protein in unpermeabilized cells ?

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Answer

The method of fixation is unlikely to have significant effect on the His-tag. However, the fixation may influence the conformation of the tagged protein, and thereby, affect the accessibility of the His-tag. Thus, one must empirically determine the influence of fixation upon ability to detect tagged proteins.

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