为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Tags & Cell Markers Epitope Tags His Tag

FITC荧光Anti-6X His tag®抗体(ab1206)

  • Datasheet
  • SDS
Reviews (7)Q&A (2)References (22)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)
  • Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)

Key features and details

  • FITC Rabbit polyclonal to 6X His tag®
  • Suitable for: ELISA, Flow Cyt
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

你可能会感兴趣

偶联
Product image
FITC Conjugation Kit - Lightning-Link® (ab102884)
一抗
DyLight® 650 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117492)
一抗
Anti-SBP Tag antibody (ab119491)

查看更多相关产品

概述

  • 产品名称

    FITC荧光Anti-6X His tag®抗体
    参阅全部 6X His tag® 一抗
  • 描述

    FITC荧光兔多克隆抗体to 6X His tag®
  • 宿主

    Rabbit
  • 偶联物

    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用

    适用于: ELISA, Flow Cytmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Synthetic peptide: HHHHHH conjugated to KLH.

  • 常规说明

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Molar F/P ratio is 3.7:1.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • His Tag

相关产品

  • Isotype control

    • FITC-Rabbit IgG - Isotype Control (ab37406)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1206于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA (1)
Use at an assay dependent concentration.
Flow Cyt (5)
Use at an assay dependent concentration.

ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

说明
ELISA
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

图片

  • Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)
    Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)Li et al PLoS One. 2018 Jan 22;13(1):e0191024. doi: 10.1371/journal.pone.0191024. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Muc1-Bi-1 and Muc1-Bi-2 can recognize Muc1 positive tumor cells.

    a). Flow cytometry analysis of Muc1-Bi-1 and Muc1-Bi-2 on Muc1-negative cell lines HePG2 and CHO and Muc1-positive cell lines, HT29. Red line indicates cells with no staining; green line indicates cells with only anti-His-FITC staining (ab1206); blue line indicates cells with positive control anti-Muc1 antibody; purple line indicates cells with Muc1-Bi-1 protein; orange line indicates cells with Muc1-Bi-2 protein.

  • Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)
    Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)This image is courtesy of an anonymous Abreview

    ab1206 staining 6X His tag® in human endometrial carcinoma cells by Flow Cytometry.

    Cells were prepared by short trypsinisation in PBS/1% FBS/0.2% sodium azide and fixed with formaldehyde. The gate included all single cells. The sample was incubated with  a 6X His tag® primary antibody for 1 hour at 4°C. ab1206 was used as the secondary. On the image, the red line represents cells stained with ab1206 alone, and the blue line is the tested sample carrying a 6XHis tag®.  The small peak to the right represents cells that bound the tested sample.

    See Abreview

实验方案

  • Flow cytometry protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (22)

发表研究结果有使用 ab1206?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab1206 被引用在 22 文献中.

  • Zhang C  et al. Novel EGFR-bispecific recombinant immunotoxin based on cucurmosin shows potent anti-tumor efficiency in vitro. Oncol Rep N/A:N/A (2020). PubMed: 33416157
  • Du F  et al. Engineering an EGFR-binding Gp2 domain for increased hydrophilicity. Biotechnol Bioeng 116:526-535 (2019). PubMed: 30536855
  • Stern LA  et al. Cellular-Based Selections Aid Yeast-Display Discovery of Genuine Cell-Binding Ligands: Targeting Oncology Vascular Biomarker CD276. ACS Comb Sci 21:207-222 (2019). PubMed: 30620189
  • Wang H  et al. Rice Yellow Stunt Nucleorhabdovirus Matrix Protein Mediates Viral Axonal Transport in the Central Nervous System of Its Insect Vector. Front Microbiol 10:939 (2019). PubMed: 31143161
  • Wang J  et al. In vitro affinity maturation of antibody against membrane-bound GPCR molecules. Appl Microbiol Biotechnol 103:7703-7717 (2019). PubMed: 31359103
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

ELISA abreview for Anti-6X His tag® antibody (FITC)

Good
Abreviews
Abreviews
Application
ELISA
Sample
Pichia pastoris Recombinant protein (yeast cell)
Specification
yeast cell
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Type
Indirect
Read More

Abcam user community

Verified customer

提交于 May 14 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2021 艾博抗(上海)贸易有限公司版权所有