重组Anti-Firefly Luciferase抗体[EPR17790] (ab185924)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17790] to Firefly Luciferase
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Firefly
Related conjugates and formulations
概述
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产品名称
Anti-Firefly Luciferase抗体[EPR17790]
参阅全部 Firefly Luciferase 一抗 -
描述
兔单克隆抗体[EPR17790] to Firefly Luciferase -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt, ICC/IF, WBmore details -
种属反应性
与反应: Firefly -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Firefly Luciferase transfected HEK293 whole cell lysate. ICC/IF: Firefly Luciferase transfected HEK293T cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17790 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab185924于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use at an assay dependent concentration.
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ICC/IF | (1) |
1/500.
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WB | (1) |
1/20000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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说明 |
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Flow Cyt
Use at an assay dependent concentration. |
ICC/IF
1/500. |
WB
1/20000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
靶标
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相关性
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. -
细胞定位
Peroxisome -
数据库链接
- SwissProt: P08659 Firefly
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别名
- ec 1.13.12.7 antibody
- Firefly antibody
- Luciferase antibody
- Luciferin 4 monooxygenase antibody
图片
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Firefly Luciferase, labeling Firefly Luciferase with ab185924 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK293T cells transfected with Firefly Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab185924 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
Flow Cytometry analysis of 293T (Human epithelial cell line from embryonic kidney) cells transfected with GFP tagged Firefly Luciferase labeled with purified ab185924 at 1/70 dilution (10µg/ml, Right panel). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 647)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Left panel) was used as the isotype control.
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All lanes : Anti-Firefly Luciferase antibody [EPR17790] (ab185924) at 1/20000 dilution
Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Firefly Luciferase antibody [EPR17790] (ab185924) at 1/1000 dilution
Lane 1 : Firefly Luciferase transfected HEK293 (human embryonic kidney) whole cell lysate at 10 µg
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
Lane 5 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg
Lane 6 : Human fetal brain lysate at 10 µg
Lane 7 : Human fetal heart lysate at 10 µg
Lane 8 : Human fetal kidney lysate at 10 µg
Lane 9 : Human fetal spleen lysate at 10 µg
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Firefly Luciferase antibody [EPR17790] (ab185924) at 1/1000 dilution
Lane 1 : Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Mouse spleen lysate
Lane 6 : Rat brain lysate
Lane 7 : Rat heart lysate
Lane 8 : Rat kidney lysate
Lane 9 : Rat spleen lysate
Lane 10 : C6 (Rat glial tumor cells) whole cell lysate
Lane 11 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 12 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 13 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (13)
ab185924 被引用在 13 文献中.
- Zhu J et al. Nucleolin interacts with the rabbit hemorrhagic disease virus replicase RdRp, nonstructural proteins p16 and p23, playing a role in virus replication. Virol Sin 37:48-59 (2022). PubMed: 35234629
- Fu PF et al. CRISPR/Cas9-based generation of a recombinant double-reporter pseudorabies virus and its characterization in vitro and in vivo. Vet Res 52:95 (2021). PubMed: 34174954
- Zhang C et al. GDF11 enhances therapeutic functions of mesenchymal stem cells for angiogenesis. Stem Cell Res Ther 12:456 (2021). PubMed: 34384486
- Jiang Y et al. Exosomal long noncoding RNA HOXD-AS1 promotes prostate cancer metastasis via miR-361-5p/FOXM1 axis. Cell Death Dis 12:1129 (2021). PubMed: 34864822
- Chen S et al. Endogenous hydrogen sulfide regulates xCT stability through persulfidation of OTUB1 at cysteine 91 in colon cancer cells. Neoplasia 23:461-472 (2021). PubMed: 33878705