Application
Western blot
Sample
Human Cell lysate - other (HeLa cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
2.89 µg
Specification
HeLa cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/10000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG(H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 280.6 kDa
Additional data
Additional Notes
I centrifuged the cell lysate, and made the SDS-PAGE sample with supernatant.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 16 2021