Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hela (human) and 3t3 (mouse))
Loading amount
154 µg
Specification
Hela (human) and 3t3 (mouse)
Gel Running Conditions
Reduced Denaturing (8% sds page)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Antirabbit HRP conjugated from Pierce
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 280, 180.120 kDa
Additional data
Additional Notes
Hela (Hu) and 3t3 (ms), respetively
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 15 2009