Anti-FIF抗体(ab99307)
Key features and details
- Rabbit polyclonal to FIF
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-FIF抗体
参阅全部 FIF 一抗 -
描述
兔多克隆抗体to FIF -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus -
免疫原
Synthetic peptide corresponding to Human FIF aa 1-50.
Database link: XP_508375.2 -
阳性对照
- Whole cell lysates from HeLa, 293T and Mouse NIH 3T3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99307于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 58 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. -
组织特异性
Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer. -
序列相似性
Belongs to the API5 family. -
结构域
Two regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2. -
细胞定位
Cytoplasm and Nucleus. Cytoplasm. Mainly nuclear. Can also be cytoplasmic. - Information by UniProt
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数据库链接
- Entrez Gene: 8539 Human
- Entrez Gene: 11800 Mouse
- Omim: 609774 Human
- SwissProt: Q9BZZ5 Human
- SwissProt: O35841 Mouse
- Unigene: 435771 Human
- Unigene: 181824 Mouse
- Unigene: 402557 Mouse
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别名
- AAC 11 antibody
- AAC-11 antibody
- AAC11 antibody
see all
图片
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All lanes : Anti-FIF antibody (ab99307) at 0.04 µg/ml
Lane 1 : HeLa whole cell
lysate at 50 µg
Lane 2 : HeLa whole cell
lysate at 15 µg
Lane 3 : HeLa whole cell
lysate at 5 µg
Lane 4 : 293T whole cell
lysate at 50 µg
Lane 5 : NIH 3T3 whole cell
lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling FIF with ab99307 at 1/1000 (0.2µg/mg) and 1/200 (1 µg/mg). Detection: DAB.
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ab99307, at 0.4 µg/ml, staining FIF in HeLa whole cell lysate immunoprecipitated using ab99307 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 1 second.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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