概述

  • 产品名称

    Anti-Fibronectin抗体
    参阅全部 Fibronectin 一抗
  • 描述

    兔多克隆抗体to Fibronectin
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, WB, IP, IHC-FoFr, IHC-P, IHC-Frmore details
  • 种属反应性

    与反应: Mouse, Rat, Hamster, Cow, Dog, Human, African green monkey, Chinese hamster, Syrian hamster
  • 免疫原

    Fibronectin isolated from a pool of normal human plasma.

  • 阳性对照

    • IHC-Fr: Mouse colon and embryonic heart tissue. IHC-P: Human kidney tissue. WB: Human colon lysate; HepG2 and NIH/3T3 cell lysate. ICC/IF: HeLa cells.
  • 常规说明

      

性能

应用

Our Abpromise guarantee covers the use of ab2413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 285 kDa (predicted molecular weight: 262 kDa).
IP Use at an assay dependent concentration.

Used at 1ug/ml for 1 hr 30 min on renal carcinoma cell line 786-0 (see Abreview).

IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

This antibody may be diluted to a titer of 1/50 - 1/250 in an ABC method. We suggest an incubation period of 30 minutes at room temperature.

靶标

  • 功能

    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • 组织特异性

    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • 疾病相关

    Glomerulopathy with fibronectin deposits 2
  • 序列相似性

    Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • 发展阶段

    Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • 翻译后修饰

    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • 细胞定位

    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 数据库链接

  • 别名

    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • Fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Ugl-Y3 antibody
    see all

图片

  • Paraformaldehyde-fixed frozen section of mouse colon tissue stained for Fibronectin (green) using ab2413 at 1/200 dilution in immunohistochemical analysis, followed by Alexa Fluor® 488 conjugated Goat anti-Rabbit IgG (H+L).

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, staining Fibronectin with ab2413.
  • All lanes : Anti-Fibronectin antibody (ab2413) at 1 µg/ml

    Lane 1 : Human colon tissue lysate - total protein (ab30051)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 262 kDa
    Observed band size: 285 kDa
    why is the actual band size different from the predicted?

  • Paraformaldehyde-fixed frozen section of mouse embryonic heart tissue stained for Fibronectin (green) using ab2413 at 1/100 dilution in immunohistochemical analysis, followed by Alexa Fluor® 488 conjugated Donkey anti-Rabbit IgG.

    Nuclear counterstain: DAPI (blue).

    See Abreview

  • ICC/IF image of ab2413 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2413, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
  • ab2413 staining Fibronectin in human kidney tissue section by IHC-P (Formalin/PFA-fixed paraffin embedded tissue sections). Tissue sections were incubated with ab2413 at a dilution of 1:250 for one hour. Heat mediated antigen retrieval technique was used with citrate buffer at pH 6.0. DAB staining was done with a biotinylated secondary for 45 min at RT at a concentration of 1:1000.

文献

This product has been referenced in:

  • Murphy S  et al. Proteomic analysis of the sarcolemma-enriched fraction from dystrophic mdx-4cv skeletal muscle. J Proteomics 191:212-227 (2019). Read more (PubMed: 29408692) »
  • Dye DE  et al. Interaction Between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System. Methods Mol Biol 1889:185-212 (2019). Read more (PubMed: 30367415) »
See all 255 Publications for this product

客户评价及客户问答

1-10 of 80 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryo)
Permeabilization
Yes - 0.05% tritonX-100
Specification
embryo
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Radhika Atit

Verified customer

提交于 Jul 10 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Prostate
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Mr. David Ivancic

Verified customer

提交于 Jul 01 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (heart)
Permeabilization
No
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Jun 21 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (Human Melanoma)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
15 µg
Specification
Human Melanoma
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 May 02 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (murine Breast cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9
Permeabilization
Yes - TBS-T
Specification
murine Breast cancer
Blocking step
1% BSA+ 1%FBS in TBS-T as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Feb 15 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (murine Breast cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9
Permeabilization
Yes - TBS-T
Specification
murine Breast cancer
Blocking step
BSA _ FBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: Rt°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Feb 15 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (BJ fibroblasts)
Gel Running Conditions
Non-reduced Denaturing (7.5%)
Loading amount
5 µg
Treatment
5ng/mL TGF-b for 24hrs
Specification
BJ fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Feb 12 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Vascular smooth muscle cell)
Permeabilization
No
Specification
Vascular smooth muscle cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Dec 12 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (Vascular smooth muscle cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Vascular smooth muscle cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

提交于 Dec 12 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse colon tissue)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
20 µg
Specification
mouse colon tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Sep 11 2018

1-10 of 80 Abreviews or Q&A

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