Application
Flow Cytometry
Sample
Human Cell (platelets (platelet rich plasma))
Permeabilization
No
Gating Strategy
platelets were gated
Specification
platelets (platelet rich plasma)
Preparation
Cell harvesting/tissue preparation method: apheresis platelet product, activated with ristocetin.
Sample buffer: HEPES-Tyrode's, pH 7.2
Sample buffer: HEPES-Tyrode's, pH 7.2
Fixation
none
Other product details
Incubation time
20 minute(s) · Temperature: 22°C · Diluent: HEPES-Tyrode buffer, pH 7.3
Dilution
1/100
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Platelets (200x10E6/ml) were activated with ristocetin (3mg/ml, 10min, 37C). Following activation, sample was diluted to 20x10E6/ml and Fibrinogen antibody was added.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dianne Van Der Wal
Verified customer
提交于 Jul 12 2016