Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Sample
Xenopus laevis Cell lysate - nuclear (adult heart)
Specification
adult heart
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk/TBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Bands
Specific: 30 kDa
Exposure
10 minute(s) and 0 second(s)
Negative control
Cytoplasmic sample was blank
Positive control
None
Additional data
Additional Notes
The three lines represent the ladder (50, 37, 25kDa), and the lane is Xenopus laevis extract. No other bands were seen on the blot
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 20 2014