Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - .25% triton x-100 10min RT
Specification
HeLa
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 3% BSA PBS
Dilution
1/400
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG Alexa Fluor 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Additional data
Additional Notes
Also tried on Methanol fixed and Acetone permeabilized HeLa cells. Worked great although the signal is better for the PFA fixed cells. The signal to noise ratio is excellent - very low background and high signal intensity. Can be diluted more.
NEW: submitted image
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提交于 Nov 29 2010