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Tags & Cell Markers Subcellular Markers Nucleus Nucleolus

Anti-Fibrillarin抗体- Nucleolar Marker (ab5821)

  • Datasheet
  • SDS
Reviews (21)Q&A (6)References (140)

Product price, shipping and contact information

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Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody - Nucleolar Marker (ab5821)
  • Western blot - Anti-Fibrillarin antibody - Nucleolar Marker (ab5821)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody - Nucleolar Marker (ab5821)

Key features and details

  • Rabbit polyclonal to Fibrillarin - Nucleolar Marker
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human, Drosophila melanogaster
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Fibrillarin抗体- Nucleolar Marker
    参阅全部 Fibrillarin 一抗
  • 描述

    兔多克隆抗体to Fibrillarin - Nucleolar Marker
  • 宿主

    Rabbit
  • 特异性

    This antibody detects a band at close to 34kDa in all species tested. The band can be completely blocked with the immuising peptide in all cases - this is very strong evidence that the antibody is recognising fibrillarin.
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Mouse, Human, Drosophila melanogaster
    预测可用于: Xenopus laevis不与反应: Rat
  • 免疫原

    Synthetic peptide corresponding to Human Fibrillarin aa 1-100 (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P22087
    (Peptide available as ab13745)

  • 阳性对照

    • ICC/IF: HeLa cells
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nucleolus
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Fibrillarin protein (ab99443)
  • Related Products

    • Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence - Cytopainter (ab139474)
    • Nucleolar Staining Kit - Green Fluorescence - Cytopainter (ab139475)

应用

Our Abpromise guarantee covers the use of ab5821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 0.1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 34 kDa).

靶标

  • 功能

    S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
  • 序列相似性

    Belongs to the methyltransferase superfamily. Fibrillarin family.
  • 翻译后修饰

    By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).
  • 细胞定位

    Nucleus, nucleolus. Fibrillar region of the nucleolus.
  • Target information above from: UniProt accession P22087 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 37662 Drosophila melanogaster
    • Entrez Gene: 2091 Human
    • Entrez Gene: 102643269 Mouse
    • Entrez Gene: 14113 Mouse
    • Omim: 134795 Human
    • SwissProt: Q9W1V3 Drosophila melanogaster
    • SwissProt: P22087 Human
    • SwissProt: P35550 Mouse
    • SwissProt: P22232 Xenopus laevis
    • Unigene: 2821 Drosophila melanogaster
    • Unigene: 299002 Human
    • Unigene: 4595 Mouse
    • Unigene: 8139 Xenopus laevis
    see all
  • 别名

    • 34 kD nucleolar scleroderma antigen antibody
    • 34 kDa nucleolar scleroderma antigen antibody
    • Fbl antibody
    • FBRL_HUMAN antibody
    • FIB antibody
    • FIB1 antibody
    • FLRN antibody
    • Histone-glutamine methyltransferase antibody
    • Nop1p antibody
    • RNA U3 small nucleolar interacting protein 1 antibody
    • RNU3IP1 antibody
    • rRNA 2' O methyltransferase fibrillarin antibody
    • rRNA 2'-O-methyltransferase fibrillarin antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody - Nucleolar Marker (ab5821)
    Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody - Nucleolar Marker (ab5821)Lab

    ab5821 staining Fibrillarin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab5821 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  • Western blot - Anti-Fibrillarin antibody - Nucleolar Marker (ab5821)
    Western blot - Anti-Fibrillarin antibody - Nucleolar Marker (ab5821)
    All lanes : Anti-Fibrillarin antibody - Nucleolar Marker (ab5821) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Schneider L2 whole cell lysate (ab14893)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 42 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody - Nucleolar Marker (ab5821)
    Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody - Nucleolar Marker (ab5821)This image is courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK

    Immunofluorescent imaging of human cells (U2OS) with ab5821 reveals highly specific localisation to the dense fibrillar component (DFC) of the nucleolus associated with the initial ribosomal RNA (rRNA) precursor. The nucleolar protein fibrillarin is located primarily in the DFC.  Blue is hoechst staining of the nucleus, green is ab5821 used at 1/100, merge image demonstrates exclusively nuclear localisation.  

    IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody Alexa 488 for 30 minutes.  All blocking and incubation steps carried out at 37 degrees C.

实验方案

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (140)

    发表研究结果有使用 ab5821?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5821 被引用在 140 文献中.

    • Martínez Corrales G  et al. Partial Inhibition of RNA Polymerase I Promotes Animal Health and Longevity. Cell Rep 30:1661-1669.e4 (2020). PubMed: 32049000
    • Gershman BW  et al. Tissue-specific Expression of Ribosomal Protein Paralogue eRpL22-like in Drosophila melanogaster Eye Development. Dev Dyn N/A:N/A (2020). PubMed: 32353187
    • Watson SF  et al. ILF3 contributes to the establishment of the antiviral type I interferon program. Nucleic Acids Res 48:116-129 (2020). PubMed: 31701124
    • Alexandrova J  et al. Full-length NF-?B repressing factor contains an XRN2 binding domain. Biochem J 477:773-786 (2020). PubMed: 32011671
    • Chen L  et al. Loss of Human TGS1 Hypermethylase Promotes Increased Telomerase RNA and Telomere Elongation. Cell Rep 30:1358-1372.e5 (2020). PubMed: 32023455
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 27 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Fibrillarin antibody - Nucleolar Marker

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rhynchosciara americana Cell (salivary gland)
    Permeabilization
    No
    Specification
    salivary gland
    Fixative
    Formaldehyde
    Read More

    Dr. Eduardo Gorab

    Verified customer

    提交于 Jul 02 2018

    Western blot abreview for Anti-Fibrillarin antibody - Nucleolar Marker

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Whole brain tissue lysate)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (3-8% Tris-Acetate)
    Loading amount
    15 µg
    Specification
    Whole brain tissue lysate
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 10 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Fibrillarin antibody - Nucleolar Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monkey Cell (Kidney)
    Permeabilization
    Yes - 0.1% triton x-100
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 10 2016

    Western blot abreview for Anti-Fibrillarin antibody - Nucleolar Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 04 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Fibrillarin antibody - Nucleolar Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3% Triton x 100: 30 min
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 May 04 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Fibrillarin antibody - Nucleolar Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse embryonic stem cells (E14 mES))
    Permeabilization
    Yes - 0.25% triton
    Specification
    mouse embryonic stem cells (E14 mES)
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 05 2016

    Western blot abreview for Anti-Fibrillarin antibody - Nucleolar Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Brain)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    Brain
    Blocking step
    Odyssey Blocking Buffer (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 45% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 18 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Fibrillarin antibody - Nucleolar Marker

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Tween-20
    Specification
    Brain
    Blocking step
    Heat-Inactivated Normal Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 27 2015

    Western blot abreview for Anti-Fibrillarin antibody - Nucleolar Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Xenopus laevis Cell lysate - nuclear (adult heart)
    Specification
    adult heart
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 20 2014

    Immunocytochemistry abreview for Anti-Fibrillarin antibody - Nucleolar Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Blocking step
    Innovex Background buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Sample
    Human Cultured Cells (Pancreatic cancer cell line)
    Specification
    Pancreatic cancer cell line
    Permeabilization
    Yes - 0.25% tritonx X-100
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 May 02 2014

    1-10 of 27 Abreviews or Q&A

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