Application
Western blot
Sample
Human Cell lysate - whole cell (Cancer cell lines)
Loading amount
15 µg
Specification
Cancer cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/200
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Positive control
DU145 (human prostate cancer cell lines), MCF7 (human breast adenocarcinoma cell line) and GN11 (murine cell line of immortalized gonadotropin-releasing hormone-expressing neurons) stably transfected with the human FGFR1 gene
Negative control
GN11 non transfected and GN11 stably transfected with the empty vector
Additional data
Additional Notes
Lane 1: GN11 cell lysates (non transfected)
Lane 2: GN11 cell lysates stably transfected with the empty vector (mock)
Lane 3: GN11 cell lysates stably transfected with the human FGFR1 gene
Lane 4: DU145 cell lysate
Lane 5: MCF7 cell lysate
Lane 2: GN11 cell lysates stably transfected with the empty vector (mock)
Lane 3: GN11 cell lysates stably transfected with the human FGFR1 gene
Lane 4: DU145 cell lysate
Lane 5: MCF7 cell lysate
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Valentina André
Verified customer
提交于 Sep 28 2012