Anti-FGD4抗体(ab97785)
Key features and details
- Rabbit polyclonal to FGD4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FGD4抗体 -
描述
兔多克隆抗体to FGD4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a sequence within amino acids 49-297 of Human FGD4 (NP_640334).
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阳性对照
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; SG xenograft tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97785于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 87 kDa.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 87 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Activates CDC42, a member of the Ras-like family of Rho-and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. Activates MAPK8. -
组织特异性
Expressed in different tissues, including brain, cerebellum, peripheral nerve, skeletal muscle, heart, uterus, placenta and testis. -
疾病相关
Charcot-Marie-Tooth disease 4H (CMT4H) [MIM:609311]: A recessive demyelinating form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Demyelinating neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4. Note=The disease is caused by mutations affecting the gene represented in this entry. -
序列相似性
Contains 1 DH (DBL-homology) domain.
Contains 1 FYVE-type zinc finger.
Contains 2 PH domains. -
结构域
The part of the protein spanning the actin filament-binding domain together with the DH domain and the first PH domain is necessary and sufficient for microspike formation. Activation of MAPK8 requires the presence of all domains with the exception of the actin filament-binding domain. -
细胞定位
Cytoplasm > cytoskeleton. Cell projection > filopodium. Concentrated in filopodia and poorly detected at lamellipodia. Binds along the sides of actin fibers. - Information by UniProt
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数据库链接
- Entrez Gene: 121512 Human
- Omim: 611104 Human
- SwissProt: Q96M96 Human
- Unigene: 117835 Human
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别名
- 9030023J02Rik antibody
- 9330209B17Rik antibody
- Actin filament binding protein frabin antibody
see all
图片
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Anti-FGD4 antibody (ab97785) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 87 kDa
7.5% SDS-PAGE. -
ab97785 at 1/200 dilution staining FGD4 in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.
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ab97785 at 1/100 dilution staining FGD4 in SG xenograft by Immunohistochemistry, Paraffin-embedded tissue.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab97785 被引用在 4 文献中.
- Lin J et al. MiR-23a induced the activation of CDC42/PAK1 pathway and cell cycle arrest in human cov434 cells by targeting FGD4. J Ovarian Res 13:90 (2020). PubMed: 32772928
- Brooks AB et al. MYO6 is targeted bySalmonellavirulence effectors to trigger PI3-kinase signaling and pathogen invasion into host cells. Proc Natl Acad Sci U S A 114:3915-3920 (2017). PubMed: 28348208
- Cao M et al. Mechanisms of Impaired Neutrophil Migration by MicroRNAs in Myelodysplastic Syndromes. J Immunol 198:1887-1899 (2017). PubMed: 28130497
- Ottman R et al. MicroRNA expressions associated with progression of prostate cancer cells to antiandrogen therapy resistance. Mol Cancer 13:1 (2014). WB ; Human . PubMed: 24387052