Anti-FEM 1B抗体(ab3686)

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Key features and details

  • Rabbit polyclonal to FEM 1B
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-FEM 1B抗体

  • 描述

    兔多克隆抗体to FEM 1B

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IF, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide:

    DINYQDQIPRTLEE

    , corresponding to amino acids 609-622 of Human FEM 1B(Peptide available as ab6098.)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    Fas and Tumor Necrosis Factor Receptor 1 (TNFR 1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR 1 was recently identified and designated F1A alpha and FEM 1 beta. F1Aa/FEM1b is the homologue of C. elegans sex determining protein FEM 1. FEM 1/F1Aa is cleaved by CED-3 and caspase. FEM 1/F1Aa associates with CED-4 and its mammalian homologue Apaf 1. Overexpression of F1Aa induces apoptosis. F1Aa is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aa is expressed in a variety of human and mouse tissues.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • Primary antibody说明

    Fas and Tumor Necrosis Factor Receptor 1 (TNFR 1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR 1 was recently identified and designated F1A alpha and FEM 1 beta. F1Aa/FEM1b is the homologue of C. elegans sex determining protein FEM 1. FEM 1/F1Aa is cleaved by CED-3 and caspase. FEM 1/F1Aa associates with CED-4 and its mammalian homologue Apaf 1. Overexpression of F1Aa induces apoptosis. F1Aa is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aa is expressed in a variety of human and mouse tissues.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 70 kDa.
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能

    Component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. Functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1.

  • 组织特异性

    Widely expressed. Highly expressed in testis. Weakly expressed in other tissues.

  • 通路

    Protein modification; protein ubiquitination.

  • 序列相似性

    Belongs to the fem-1 family.
    Contains 8 ANK repeats.
    Contains 1 TPR repeat.

  • 细胞定位

    Cytoplasm. Nucleus. In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment. Associated with chromatin.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • F1A alpha antibody
    • F1A-alpha antibody
    • F1AA antibody
    • Fem-1-like death receptor-binding protein alpha antibody
    • Fem-1-like in apoptotic pathway protein alpha antibody
    • Fem1 homolog b antibody
    • FEM1 like death receptor binding protein antibody
    • FEM1-beta antibody
    • FEM1b antibody
    • FEM1B_HUMAN antibody
    • Protein fem-1 homolog B antibody
    see all

图片

  • Western blot - Anti-FEM 1B antibody (ab3686)
    Western blot - Anti-FEM 1B antibody (ab3686)

    Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with anti-F1A alpha (ab3686) at 1 µg /ml.

    Western blot analysis of FEM 1B in mouse (A) and rat (B) liver tissue lysates with anti-FEM 1B (ab3686) at 1 µg /ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEM 1B antibody (ab3686)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEM 1B antibody (ab3686)
    Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse liver tissue labeling FEM 1B with Anti-FEM 1B antibody (ab3686) at 20μg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEM 1B antibody (ab3686)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEM 1B antibody (ab3686)

    ab3686 at 5µg/ml staining of FEM 1B in mouse liver tissue by IHC

  • Immunocytochemistry/ Immunofluorescence - Anti-FEM 1B antibody (ab3686)
    Immunocytochemistry/ Immunofluorescence - Anti-FEM 1B antibody (ab3686)
    Immunofluorescence of F1A alpha in Mouse Liver cells using ab3686 at 20 ug/ml.

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