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Cell Biology Apoptosis Receptors Death Receptors

Anti-FEM 1B抗体(ab3686)

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Western blot - Anti-FEM 1B antibody (ab3686)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEM 1B antibody (ab3686)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEM 1B antibody (ab3686)
  • Immunocytochemistry/ Immunofluorescence - Anti-FEM 1B antibody (ab3686)

Key features and details

  • Rabbit polyclonal to FEM 1B
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-FEM 1B抗体
  • 描述

    兔多克隆抗体to FEM 1B
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    DINYQDQIPRTLEE

    , corresponding to amino acids 609-622 of Human FEM 1B(Peptide available as ab6098.)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Fas and Tumor Necrosis Factor Receptor 1 (TNFR 1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR 1 was recently identified and designated F1A alpha and FEM 1 beta. F1Aa/FEM1b is the homologue of C. elegans sex determining protein FEM 1. FEM 1/F1Aa is cleaved by CED-3 and caspase. FEM 1/F1Aa associates with CED-4 and its mammalian homologue Apaf 1. Overexpression of F1Aa induces apoptosis. F1Aa is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aa is expressed in a variety of human and mouse tissues.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    Fas and Tumor Necrosis Factor Receptor 1 (TNFR 1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR 1 was recently identified and designated F1A alpha and FEM 1 beta. F1Aa/FEM1b is the homologue of C. elegans sex determining protein FEM 1. FEM 1/F1Aa is cleaved by CED-3 and caspase. FEM 1/F1Aa associates with CED-4 and its mammalian homologue Apaf 1. Overexpression of F1Aa induces apoptosis. F1Aa is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aa is expressed in a variety of human and mouse tissues.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Receptors
    • Death Receptors
    • Cell Biology
    • Apoptosis
    • Receptors
    • Associated Proteins
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Associated Proteins

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

Our Abpromise guarantee covers the use of ab3686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 70 kDa.
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能

    Component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. Functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1.
  • 组织特异性

    Widely expressed. Highly expressed in testis. Weakly expressed in other tissues.
  • 通路

    Protein modification; protein ubiquitination.
  • 序列相似性

    Belongs to the fem-1 family.
    Contains 8 ANK repeats.
    Contains 1 TPR repeat.
  • 细胞定位

    Cytoplasm. Nucleus. In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment. Associated with chromatin.
  • Target information above from: UniProt accession Q9UK73 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 10116 Human
    • Entrez Gene: 14155 Mouse
    • Entrez Gene: 315745 Rat
    • Omim: 613539 Human
    • SwissProt: Q9UK73 Human
    • SwissProt: Q9Z2G0 Mouse
    • SwissProt: P0C6P7 Rat
    • Unigene: 362733 Human
    • Unigene: 24069 Mouse
    • Unigene: 219320 Rat
    see all
  • 别名

    • F1A alpha antibody
    • F1A-alpha antibody
    • F1AA antibody
    • Fem-1-like death receptor-binding protein alpha antibody
    • Fem-1-like in apoptotic pathway protein alpha antibody
    • Fem1 homolog b antibody
    • FEM1 like death receptor binding protein antibody
    • FEM1-beta antibody
    • FEM1b antibody
    • FEM1B_HUMAN antibody
    • Protein fem-1 homolog B antibody
    see all

图片

  • Western blot - Anti-FEM 1B antibody (ab3686)
    Western blot - Anti-FEM 1B antibody (ab3686)

    Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with anti-F1A alpha (ab3686) at 1 µg /ml.

    Western blot analysis of FEM 1B in mouse (A) and rat (B) liver tissue lysates with anti-FEM 1B (ab3686) at 1 µg /ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEM 1B antibody (ab3686)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEM 1B antibody (ab3686)
    Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse liver tissue labeling FEM 1B with Anti-FEM 1B antibody (ab3686) at 20μg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEM 1B antibody (ab3686)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEM 1B antibody (ab3686)

    ab3686 at 5µg/ml staining of FEM 1B in mouse liver tissue by IHC

  • Immunocytochemistry/ Immunofluorescence - Anti-FEM 1B antibody (ab3686)
    Immunocytochemistry/ Immunofluorescence - Anti-FEM 1B antibody (ab3686)
    Immunofluorescence of F1A alpha in Mouse Liver cells using ab3686 at 20 ug/ml.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (0)

    发表研究结果有使用 ab3686?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3686 尚未被引用在任何文献中。

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