Anti-Fc epsilon RI/FCER1A抗体[9E1] (ab54411)
Key features and details
- Mouse monoclonal [9E1] to Fc epsilon RI/FCER1A
- Suitable for: ELISA, IP, ICC/IF, IHC-P, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-Fc epsilon RI/FCER1A抗体[9E1]
参阅全部 Fc epsilon RI/FCER1A 一抗 -
描述
小鼠单克隆抗体[9E1] to Fc epsilon RI/FCER1A -
宿主
Mouse -
特异性
This antibody recognizes the human high affinity Fc receptor for IgE (Fc epsilon R1), which plays a central role in the IgE-mediated allergic response. Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two di-sulphide linked gamma subunits. Clone 9E1 specifically recognizes the extracellular D1 domain of the Fc epsilon R1 alpha chain. -
经测试应用
适用于: ELISA, IP, ICC/IF, IHC-P, Flow Cyt, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant -
克隆
单克隆 -
克隆编号
9E1 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab54411于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA | (1) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
|
IHC-P | (2) |
Use at an assay dependent concentration.
|
Flow Cyt | (1) |
Use at an assay dependent concentration.
Use 10ul of the suggested dilution to label 10^6 cells in 100 ul. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB | (1) |
Use at an assay dependent concentration.
|
说明 |
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ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. Use 10ul of the suggested dilution to label 10^6 cells in 100 ul. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
靶标
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功能
Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. -
序列相似性
Contains 2 Ig-like (immunoglobulin-like) domains. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2205 Human
- Omim: 147140 Human
- SwissProt: P12319 Human
- Unigene: 897 Human
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别名
- Fc epsilon RI alpha antibody
- Fc epsilon RI alpha chain antibody
- Fc epsilon RI alpha-chain antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab54411 被引用在 6 文献中.
- Win-Shwe TT et al. Dietary Exposure to Flame Retardant Tris (2-Butoxyethyl) Phosphate Altered Neurobehavior and Neuroinflammatory Responses in a Mouse Model of Allergic Asthma. Int J Mol Sci 23:N/A (2022). PubMed: 35054842
- Konnikova L et al. Small intestinal immunopathology and GI-associated antibody formation in hereditary alpha-tryptasemia. J Allergy Clin Immunol N/A:N/A (2021). PubMed: 33865872
- Moledina DG et al. Urine TNF-a and IL-9 for clinical diagnosis of acute interstitial nephritis. JCI Insight 4:N/A (2019). PubMed: 31092735
- Nishida K et al. Mast Cells Exhibiting Strong Cytoplasmic Staining for IgE and High Affinity IgE Receptor are Increased in IgG4-Related Disease. Sci Rep 8:4656 (2018). PubMed: 29545608
- Messingham KN et al. Human eosinophils express the high affinity IgE receptor, FceRI, in bullous pemphigoid. PLoS One 9:e107725 (2014). PubMed: 25255430
- Tsai YM et al. Functional interaction of cockroach allergens and mannose receptor (CD206) in human circulating fibrocytes. PLoS One 8:e64105 (2013). Flow Cyt ; Human . PubMed: 23734186