Anti-FBP17抗体(ab100918)

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概述

  • 产品名称
    Anti-FBP17抗体
    参阅全部 FBP17 一抗
  • 描述
    兔多克隆抗体to FBP17
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Orangutan, Platypus
  • 免疫原

    Other Immunogen Type within Human FBP17 aa 350-400. The exact sequence is proprietary.
    Database link: NP_055848.1

  • 阳性对照
    • HeLa, 293T lysates

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab100918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 71 kDa.
IP Use at 5-15 µg/mg of lysate.

靶标

  • 功能
    May act as a link between RND2 signaling and regulation of the actin cytoskeleton (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also enhances actin polymerization via the recruitment of WASL/N-WASP, which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. May be required for the lysosomal retention of FASLG/FASL.
  • 组织特异性
    Very highly expressed in the epithelial cells of the gastrointestinal tract, respiratory, reproductive and urinary systems. Also highly expressed in brown adipose tissue, cardiomyocytes, enteric ganglia and glucagon producing cells of the pancreas. Expressed in germ cells of the testis and all regions of the brain.
  • 疾病相关
    Note=A chromosomal aberration involving FNBP1 is found in acute leukemias. Translocation t(9;11)(q34;q23) with MLL. The relatively low incidence of the MLL-FNBP1 fusion protein in acute leukemia may reflect the marginal capacity of this fusion protein to induce cellular transformation.
  • 序列相似性
    Belongs to the FNBP1 family.
    Contains 1 FCH domain.
    Contains 1 REM (Hr1) repeat.
    Contains 1 SH3 domain.
  • 细胞定位
    Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Lysosome. Cytoplasmic vesicle. Cell membrane. Enriched in cortical regions coincident with F-actin. Also localizes to endocytic vesicles and lysosomes.
  • Information by UniProt
  • 数据库链接
    • 别名
      • FBP1 antibody
      • FBP17 antibody
      • Fnbp1 antibody
      • FNBP1_HUMAN antibody
      • Formin binding protein 1 antibody
      • Formin binding protein 17 antibody
      • Formin-binding protein 1 antibody
      • Formin-binding protein 17 antibody
      • hFBP17 antibody
      • KIAA0554 antibody
      • MGC126804 antibody
      see all

    图片

    • Western blot - Anti-FBP17 antibody (ab100918)
      Western blot - Anti-FBP17 antibody (ab100918)
      All lanes : Anti-FBP17 antibody (ab100918) at 0.1 µg/ml

      Lane 1 : HeLa Whole Cell Lysate at 50 µg
      Lane 2 : HeLa Whole Cell Lysate at 15 µg
      Lane 3 : HeLa Whole Cell Lysate at 5 µg
      Lane 4 : 293T Whole Cell Lysate at 50 µg

      Predicted band size: 71 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-FBP17 antibody (ab100918)
      Immunoprecipitation - Anti-FBP17 antibody (ab100918)
      ab100918 at 1 µg/ml staining FBP17 in HeLa cell lysate immunoprecipitated using ab100918 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 30 seconds

    文献

    ab100918 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab100918？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    2 reviews or Q&A

    10
    Votes: Highest First


    Je vous remercie pour votre réponse. Je vais tester le ab113961.
    Bien cordialement,

    Merci pour votre réponse.

    J'ai mis en place l'envoi d'une unité de ab113961 en remplacement du ab100918. Le numéro de commande de remplacement gratuit de ce produit est ****.
    Vous recevrez prochainement un mail de conf...

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    Human lysates
    Positive control: HeLa
    WB: many non-specific bands. weak signal at the expected MW.
    5% milk
    tried 1/2000 up to 1/5000, 2 hours RT or O/N 4ºC
    50µg protein loaded

    Merci de nous avoir contactés.

    Je suis désolé que le produit ab100918 que vous avez reçu ne fonctionne pas comme attendu.

    Comme convenu par téléphone je vous envoie les liens vers les fiches t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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