Anti-FBP17抗体(ab100918)
Key features and details
- Rabbit polyclonal to FBP17
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FBP17抗体
参阅全部 FBP17 一抗 -
描述
兔多克隆抗体to FBP17 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Orangutan, Platypus
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免疫原
Other Immunogen Type within Human FBP17 aa 350-400. The exact sequence is proprietary.
Database link: NP_055848.1 -
阳性对照
- HeLa, 293T lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab100918于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 71 kDa.
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| IP |
Use at 5-15 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 71 kDa. |
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IP
Use at 5-15 µg/mg of lysate. |
靶标
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功能
May act as a link between RND2 signaling and regulation of the actin cytoskeleton (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also enhances actin polymerization via the recruitment of WASL/N-WASP, which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. May be required for the lysosomal retention of FASLG/FASL. -
组织特异性
Very highly expressed in the epithelial cells of the gastrointestinal tract, respiratory, reproductive and urinary systems. Also highly expressed in brown adipose tissue, cardiomyocytes, enteric ganglia and glucagon producing cells of the pancreas. Expressed in germ cells of the testis and all regions of the brain. -
疾病相关
Note=A chromosomal aberration involving FNBP1 is found in acute leukemias. Translocation t(9;11)(q34;q23) with MLL. The relatively low incidence of the MLL-FNBP1 fusion protein in acute leukemia may reflect the marginal capacity of this fusion protein to induce cellular transformation. -
序列相似性
Belongs to the FNBP1 family.
Contains 1 FCH domain.
Contains 1 REM (Hr1) repeat.
Contains 1 SH3 domain. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Lysosome. Cytoplasmic vesicle. Cell membrane. Enriched in cortical regions coincident with F-actin. Also localizes to endocytic vesicles and lysosomes. - Information by UniProt
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数据库链接
- Entrez Gene: 23048 Human
- Entrez Gene: 192348 Rat
- Omim: 606191 Human
- SwissProt: Q96RU3 Human
- SwissProt: Q8R511 Rat
- Unigene: 189409 Human
- Unigene: 20445 Rat
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别名
- FBP1 antibody
- FBP17 antibody
- Fnbp1 antibody
see all
图片
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Western blot - Anti-FBP17 antibody (ab100918)All lanes : Anti-FBP17 antibody (ab100918) at 0.1 µg/ml
Lane 1 : HeLa Whole Cell Lysate at 50 µg
Lane 2 : HeLa Whole Cell Lysate at 15 µg
Lane 3 : HeLa Whole Cell Lysate at 5 µg
Lane 4 : 293T Whole Cell Lysate at 50 µg
Predicted band size: 71 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-FBP17 antibody (ab100918)ab100918 at 1 µg/ml staining FBP17 in HeLa cell lysate immunoprecipitated using ab100918 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 30 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab100918 尚未被引用在任何文献中。