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Cardiovascular Lipids / Lipoproteins Fatty Acids Synthesis
使用敲除细胞株进行验证重组RabMAb

重组Anti-Fatty Acid Synthase抗体[EPR7466] (ab128870)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (28)

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Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
  • Immunoprecipitation - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
  • Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
  • Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7466] to Fatty Acid Synthase
  • Suitable for: IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free HRP

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概述

  • 产品名称

    Anti-Fatty Acid Synthase抗体[EPR7466]
    参阅全部 Fatty Acid Synthase 一抗
  • 描述

    兔单克隆抗体[EPR7466] to Fatty Acid Synthase
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Fatty Acid Synthase aa 2450-2550 (C terminal). The exact sequence is proprietary.

  • 阳性对照

    • WB: HAP1 and A549 whole cell lysate.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 解离常数(KD)

    KD = 1.34 x 10 -10 M
    Learn more about KD
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR7466
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Synthesis
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Neuroscience
    • Neurology process
    • Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

相关产品

  • Alternative Versions

    • HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)
    • Alexa Fluor® 488 Anti-Fatty Acid Synthase antibody [EPR7466] (ab197485)
    • Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human Fatty Acid Synthase protein (ab131929)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab128870于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
1/30.
WB
1/10000. Predicted molecular weight: 273 kDa.

This antibody does not work well in liver tissue in WB application. We suggest ab128856 as an alternative. 

IHC-P
1/450. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

说明
IP
1/30.
WB
1/10000. Predicted molecular weight: 273 kDa.

This antibody does not work well in liver tissue in WB application. We suggest ab128856 as an alternative. 

IHC-P
1/450. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
  • 组织特异性

    Ubiquitous. Prominent expression in brain, lung, and liver.
  • 序列相似性

    Contains 1 acyl carrier domain.
  • 细胞定位

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P49327 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 2194 Human
    • Entrez Gene: 14104 Mouse
    • Entrez Gene: 50671 Rat
    • Omim: 600212 Human
    • SwissProt: P49327 Human
    • SwissProt: P19096 Mouse
    • SwissProt: P12785 Rat
    • Unigene: 83190 Human
    • Unigene: 236443 Mouse
    • Unigene: 9486 Rat
    see all
  • 别名

    • [Acyl-carrier-protein] S acetyltransferase antibody
    • [Acyl-carrier-protein] S malonyltransferase antibody
    • 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
    • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
    • 3-oxoacyl-[acyl-carrier-protein] synthase antibody
    • Enoyl-[acyl-carrier-protein] reductase antibody
    • FAS antibody
    • FAS_HUMAN antibody
    • FASN antibody
    • Fatty acid synthase antibody
    • MGC14367 antibody
    • MGC15706 antibody
    • OA 519 antibody
    • Oleoyl-[acyl-carrier-protein] hydrolase antibody
    • SDR27X1 antibody
    • Short chain dehydrogenase/reductase family 27X member 1 antibody
    see all

图片

  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Lanes 1-7 : Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870) at 1/1000 dilution (Purified)
    Lanes 8-9 : Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 5 : Mouse brain lysate
    Lane 6 : L6 (Rat skeletal muscle myoblast) whole cell lysate
    Lane 7 : Rat brain lysate
    Lane 8 : Rat liver lysate
    Lane 9 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 273 kDa

  • Immunoprecipitation - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Immunoprecipitation - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

    Purified ab128870 at 1:30 dilution (2µg) immunoprecipitating Fatty Acid Synthase in HEK-293 whole cell lysate.
    Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab128870 + HEK-293 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128870 in HEK-293 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:10,000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: kDa

  • Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

    Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labelling Fatty Acid Synthase with Purified ab128870 at 1:50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling Fatty Acid Synthase with Purified ab128870 at 1:450 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Fatty Acid Synthase with Purified ab128870 at 1:450 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling Fatty Acid Synthase with Purified ab128870 at 1:450 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Fatty Acid Synthase with Purified ab128870 at 1:50 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg)
    Lane 3: A549 cell lysate (20 µg)
    Lane 4: Hu liver tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab128870 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab128870 was shown to react with Fatty Acid Synthase in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab128870 and ab18058 (loading control to Vinculin) were both diluted at 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (28)

发表研究结果有使用 ab128870?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab128870 被引用在 28 文献中.

  • Tian X  et al. Construction of a robust prognostic model for adult adrenocortical carcinoma: Results from bioinformatics and real-world data. J Cell Mol Med 25:3898-3911 (2021). PubMed: 33626208
  • Lou Y  et al. Akt kinase LANCL2 functions as a key driver in EGFR-mutant lung adenocarcinoma tumorigenesis. Cell Death Dis 12:170 (2021). PubMed: 33568630
  • Gao Y  et al. Curcumin inhibits classical swine fever virus replication by interfering with lipid metabolism. Vet Microbiol 259:109152 (2021). PubMed: 34146894
  • Liu L & Lan S Interference of S100A16 suppresses lipid accumulation and inflammation in high glucose-induced HK-2 cells. Int Urol Nephrol 53:1255-1263 (2021). PubMed: 33389513
  • Tan HL  et al. Human amniotic mesenchymal stem cells-conditioned medium protects mice from high-fat diet-induced obesity. Stem Cell Res Ther 12:364 (2021). PubMed: 34174964
View all Publications for this product

客户评价及客户问答

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提交评价 提交问题

1-3 of 3 Abreviews or Q&A

Question

I am interested in testing this product
thank you

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Abcam community

Verified customer

Asked on Nov 12 2012

Answer

DISCOUNT CODE: xxxxxxxxxx
Expiration date: xxxxxxxxx
Value: $xxxxx

I am very pleased to hear you would like to accept our offer and test ab128870 in Pig. This code will give you $xxxxxxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for Pig and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Abcam Scientific Support

回复于 Nov 12 2012

Question

Do you have a FASN (fatty acid synthase) antibody that will recognize pig (sus scrofa). I attached the pig amino acid sequence for FASN. thank you

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Abcam community

Verified customer

Asked on Nov 09 2012

Answer

Thank you for contacting us.

I have looked through our catalogue and I have not found an anti-fatty acid synthase which is guarenteed to work in pig. However, I have found an antibody ab128870 (https://www.abcam.com/ab128870) who's immunogen shows 86.7% homology with that of pig and therefore it is likely that this antibody will work in pig.

Please do note that this antibody has not been tested in pig and therefore is not guarenteed to work. However, if you wish to test this antibody in pig I can offer you a testing discount.
Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Abcam Scientific Support

回复于 Nov 09 2012

Question

Nos han llegado los anticuerpos ab128870 y ab18180 y tenemos una duda acerca de su conservación. ¿Es necesario preparar el buffer o los anticuerpos están ya disueltos en él?
Muchas gracias.

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Abcam community

Verified customer

Asked on Jun 14 2012

Answer

Gracias por contactarnos.



Los anticuerpos están ya disueltos en el buffer apropiado, lo que sí es importante seguir son las instrucciones para conservarlo en perfecto estado, evitando ciclos de congelación / descongelación (preparando alícuotas) y manteniéndolo a la temperatura adecuada.

Espero haberte ayudado. Para cualquier otra consulta o sugerencia, por favor no dudes en volvernos a contactar.

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Abcam Scientific Support

回复于 Jun 14 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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