使用敲除细胞株进行验证重组RabMAb

重组Anti-Fatty Acid Synthase抗体[EPR7465] (ab128856)

Reviews (5)Q&A (1)References (11)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7465] to Fatty Acid Synthase
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

概述

  • 产品名称

    Anti-Fatty Acid Synthase抗体[EPR7465]
    参阅全部 Fatty Acid Synthase 一抗

  • 描述

    兔单克隆抗体[EPR7465] to Fatty Acid Synthase

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Fatty Acid Synthase aa 1-100. The exact sequence is proprietary.
    Database link: P49327

  • 阳性对照

    • WB: HeLa, 293T, A549, SHSY-5Y and MOLT4 cell lysates. ICC/IF: A549 cells. IHC-Fr: Human peritoneal tumor tissue. Flow Cytometry: A549 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

应用

Our Abpromise guarantee covers the use of ab128856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 273 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/250 - 1/500.
  • 应用说明
    Is unsuitable for IHC-P or IP.

    • 靶标

    • 功能

      Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.

    • 组织特异性

      Ubiquitous. Prominent expression in brain, lung, and liver.

    • 序列相似性

      Contains 1 acyl carrier domain.

    • 细胞定位

      Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • [Acyl-carrier-protein] S acetyltransferase antibody
      • [Acyl-carrier-protein] S malonyltransferase antibody
      • 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
      • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
      • 3-oxoacyl-[acyl-carrier-protein] synthase antibody
      • Enoyl-[acyl-carrier-protein] reductase antibody
      • FAS antibody
      • FAS_HUMAN antibody
      • FASN antibody
      • Fatty acid synthase antibody
      • MGC14367 antibody
      • MGC15706 antibody
      • OA 519 antibody
      • Oleoyl-[acyl-carrier-protein] hydrolase antibody
      • SDR27X1 antibody
      • Short chain dehydrogenase/reductase family 27X member 1 antibody
      see all

    图片

    • Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
      Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg)
      Lane 3: A549 cell lysate (20 µg)
      Lane 4: Hu liver tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab128856 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.

      ab128856 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab128856 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
      Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
      All lanes : Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : SHSY-5Y cell lysate
      Lane 5 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 273 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
      Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
      ab128856 at a 1/250 dilution staining Fatty Acid Synthase in A549 cells by immunofluorescence.
    • Flow Cytometry - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
      Flow Cytometry - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
      Overlay histogram showing A549 cells stained with ab128856 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128856, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • OI-RD Scanning - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
      OI-RD Scanning - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
      Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)

    文献 (11)

    发表研究结果有使用 ab128856？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab128856 被引用在 11 文献中.

    • Ma X  et al. Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs. Genes Nutr 14:23 (2019). PubMed: 31367261
    • Xue D  et al. Quantitative proteomic analysis of sperm in unexplained recurrent pregnancy loss. Reprod Biol Endocrinol 17:52 (2019). PubMed: 31288842
    • Wu Y  et al. Berberine (BBR) Attenuated Palmitic Acid (PA)-Induced Lipotoxicity in Human HK-2 Cells by Promoting Peroxisome Proliferator-Activated Receptor a (PPAR-a). Med Sci Monit 25:7702-7708 (2019). PubMed: 31607744
    • Tao XR  et al. Angiotensinogen in hepatocytes contributes to Western diet-induced liver steatosis. J Lipid Res 60:1983-1995 (2019). PubMed: 31604805
    • Carbonetti G  et al. FABP5 coordinates lipid signaling that promotes prostate cancer metastasis. Sci Rep 9:18944 (2019). PubMed: 31831821
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
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    1-6 of 6 Abreviews or Q&A

    Flow Cytometry abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Peripheral blood mononuclear cells)
    Permeabilization
    Yes - Methanol
    Gating Strategy
    Live cells.
    Specification
    Peripheral blood mononuclear cells
    Fixation
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 19 2018

    Western blot abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (rat liver)
    Gel Running Conditions
    Reduced Denaturing (7% SDS-PAGE)
    Loading amount
    20 µg
    Specification
    rat liver
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 05 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse Cell (Tumor))
    Permeabilization
    Yes - 0.3% triton x100
    Specification
    Mouse Cell (Tumor)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 05 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Sample
    Human Tissue sections (Peritoneal tumor)
    Specification
    Peritoneal tumor
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 14 2014

    Western blot abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (8% GEL, SDS PAGE)
    Sample
    Human Cell lysate - other (LS180)
    Specification
    LS180
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 01 2013

    Question

    Customer kindly contacted us regarding use of this product in Musca domestica.

    Read More
    Answer

    Thank you for contacting us.

    I have reviewed the immunogen information for each of our anti-Fatty Acid Synthase antibodies. Unfortunately none have homologies which would guarantee they could work in Musca domestica, however ab128856 Rabbit monoclonal [EPR7465] to Fatty Acid Synthase does have an 87% positive identity score. Further the identical residues are grouped, which would be necessary for epitope recognition. Since the product has not yet been tested in house fly, we cannot guarantee that it will work, however, for UNTESTED applications; we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested. The terms and conditions of this offer can be found here: https://www.abcam.com/AbTrial


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

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