重组Anti-Fatty Acid Synthase抗体[EPR7465] (ab128856)
![Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.cn/ps/products/128/ab128856/Images/ab128856-260969-anti-fatty-acid-synthase-antibody-epr7465-western-blot.jpg "Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7465] to Fatty Acid Synthase
- Suitable for: WB, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Human
概述
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产品名称
Anti-Fatty Acid Synthase抗体[EPR7465]
参阅全部 Fatty Acid Synthase 一抗 -
描述
兔单克隆抗体[EPR7465] to Fatty Acid Synthase -
宿主
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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免疫原
Synthetic peptide within Human Fatty Acid Synthase aa 1-100. The exact sequence is proprietary.
Database link: P49327 -
阳性对照
- WB: HeLa, 293T, A549, SHSY-5Y and MOLT4 cell lysates. ICC/IF: A549 cells. IHC-Fr: Human peritoneal tumor tissue. Flow Cytometry: A549 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.58 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7465 -
同种型
IgG -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 273 kDa.
|
| Flow Cyt | (1) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
| ICC/IF | (1) |
1/250 - 1/500.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 273 kDa. |
|
Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
|
ICC/IF
1/250 - 1/500. |
靶标
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功能
Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. -
组织特异性
Ubiquitous. Prominent expression in brain, lung, and liver. -
序列相似性
Contains 1 acyl carrier domain. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 2194 Human
- Entrez Gene: 14104 Mouse
- Entrez Gene: 50671 Rat
- Omim: 600212 Human
- SwissProt: P49327 Human
- SwissProt: P19096 Mouse
- SwissProt: P12785 Rat
- Unigene: 83190 Human
see all -
别名
- [Acyl-carrier-protein] S acetyltransferase antibody
- [Acyl-carrier-protein] S malonyltransferase antibody
- 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
see all
图片
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Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: Hu liver tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab128856 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.ab128856 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab128856 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.cn/ps/products/128/ab128856/Images/ab128856-142613-anti-fatty-acid-synthase-antibody-epr7465-western-blot.jpg)
Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)All lanes : Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : SHSY-5Y cell lysate
Lane 5 : MOLT4 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 273 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.cn/ps/products/128/ab128856/Images/ab128856-142612-anti-fatty-acid-synthase-antibody-epr7465-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)ab128856 at a 1/250 dilution staining Fatty Acid Synthase in A549 cells by immunofluorescence. -
![Flow Cytometry - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.cn/ps/products/128/ab128856/Images/ab128856-238332-anti-fatty-acid-synthase-antibody-epr7465-flow-cytometry.jpg)
Flow Cytometry - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)Overlay histogram showing A549 cells stained with ab128856 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128856, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![OI-RD Scanning - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.cn/ps/products/128/ab128856/Images/ab128856-142611-anti-fatty-acid-synthase-antibody-epr7465.jpg)
OI-RD Scanning - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856) -
![Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.cn/ps/products/128/ab128856/Images/ab128856-7-benefits-of-recombinant-antibodies.png)
Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab128856 被引用在 11 文献中.
- Ma X et al. Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs. Genes Nutr 14:23 (2019). PubMed: 31367261
- Xue D et al. Quantitative proteomic analysis of sperm in unexplained recurrent pregnancy loss. Reprod Biol Endocrinol 17:52 (2019). PubMed: 31288842
- Wu Y et al. Berberine (BBR) Attenuated Palmitic Acid (PA)-Induced Lipotoxicity in Human HK-2 Cells by Promoting Peroxisome Proliferator-Activated Receptor a (PPAR-a). Med Sci Monit 25:7702-7708 (2019). PubMed: 31607744
- Tao XR et al. Angiotensinogen in hepatocytes contributes to Western diet-induced liver steatosis. J Lipid Res 60:1983-1995 (2019). PubMed: 31604805
- Carbonetti G et al. FABP5 coordinates lipid signaling that promotes prostate cancer metastasis. Sci Rep 9:18944 (2019). PubMed: 31831821