Anti-Fatty Acid Synthase抗体(ab99359)
Key features and details
- Rabbit polyclonal to Fatty Acid Synthase
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Fatty Acid Synthase抗体
参阅全部 Fatty Acid Synthase 一抗 -
描述
兔多克隆抗体to Fatty Acid Synthase -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster -
免疫原
A region within amino acids 2462-2511 of Human Fatty Acid Synthase (NP_004095.4).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab99359 was affinity purified using an epitope specific to Fatty Acid Synthase immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99359于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 273 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Predicted molecular weight: 273 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. -
组织特异性
Ubiquitous. Prominent expression in brain, lung, and liver. -
序列相似性
Contains 1 acyl carrier domain. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 281152 Cow
- Entrez Gene: 2194 Human
- Entrez Gene: 14104 Mouse
- Entrez Gene: 50671 Rat
- Omim: 600212 Human
- SwissProt: Q71SP7 Cow
- SwissProt: P08757 Goat
- SwissProt: P49327 Human
see all -
别名
- [Acyl-carrier-protein] S acetyltransferase antibody
- [Acyl-carrier-protein] S malonyltransferase antibody
- 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
see all
图片
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IHC image of Fatty Acid Synthase staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99359, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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ab99359 at 0.1 µg/ml detecting Fatty Acid Synthase in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 30 second exposure.
For immunoprecipitation, ab99359 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
Lane 1; IP using ab99358 (a rabbit anti-Fatty Acid Synthase antibody which recognizes a upstream epitope).
Lane 2; IP using ab99359.
Lane 3; IP using control IgG. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling Fatty Acid Synthase with ab99359 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-Fatty Acid Synthase antibody (ab99359) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 273 kDa
Exposure time: 30 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab99359 被引用在 12 文献中.
- Raab S et al. Evaluation of the expression of fatty acid synthase and O-GlcNAc transferase in patients with liver cancer by exploration of transcriptome databases and experimental approaches. Oncol Lett 23:105 (2022). PubMed: 35242233
- Khiewkamrop P et al. Epigallocatechin gallate triggers apoptosis by suppressing de novo lipogenesis in colorectal carcinoma cells. FEBS Open Bio 12:937-958 (2022). PubMed: 35243817
- Wang L et al. Mechanism of Astragalus membranaceus Alleviating Acquired Hyperlipidemia Induced by High-Fat Diet through Regulating Lipid Metabolism. Nutrients 14:N/A (2022). PubMed: 35267929
- Wang J et al. Altered Liver Metabolism, Mitochondrial Function, Oxidative Status, and Inflammatory Response in Intrauterine Growth Restriction Piglets with Different Growth Patterns before Weaning. Metabolites 12:N/A (2022). PubMed: 36355136
- Lin J et al. Hypoxia-induced miR-27 and miR-195 regulate ATP consumption, viability, and metabolism of rat cardiomyocytes by targeting PPAR? and FASN expression. Aging (Albany NY) 13:10158-10174 (2021). PubMed: 33819184