Anti-FANCC抗体(ab97575)
Key features and details
- Rabbit polyclonal to FANCC
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-FANCC抗体
参阅全部 FANCC 一抗 -
描述
兔多克隆抗体to FANCC -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 1 and 275 of FANCC (Q00597).
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阳性对照
- WB: H1299, NIH 3T3, 293T, A431, HepG2 or Raji cell lysate; ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97575于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 63 kDa.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 63 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Upon IFNG induction, may facilitate STAT1 activation by recruiting STAT1 to IFNGR1. -
组织特异性
Ubiquitous. -
疾病相关
Defects in FANCC are the cause of Fanconi anemia complementation group C (FANCC) [MIM:227645]. A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. -
发展阶段
Expression increases during S phase, is maximal at the G2/M transition, and declines during M phase (at protein level). -
细胞定位
Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic. - Information by UniProt
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数据库链接
- Entrez Gene: 2176 Human
- Entrez Gene: 14088 Mouse
- Omim: 613899 Human
- SwissProt: Q00597 Human
- SwissProt: P50652 Mouse
- Unigene: 494529 Human
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别名
- bA80I15.1 antibody
- FA 3 antibody
- FA3 antibody
see all
图片
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Anti-FANCC antibody (ab97575) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 63 kDa
7.5% SDS Page -
Anti-FANCC antibody (ab97575) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 63 kDa
7.5% SDS Page -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97575 at 1/200 dilution. Bottom picture shows stain merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab97575 被引用在 2 文献中.
- Richardson CD et al. CRISPR-Cas9 genome editing in human cells occurs via the Fanconi anemia pathway. Nat Genet 50:1132-1139 (2018). PubMed: 30054595
- Shen C et al. Regulation of FANCD2 by the mTOR pathway contributes to the resistance of cancer cells to DNA double-strand breaks. Cancer Res 73:3393-401 (2013). PubMed: 23633493