Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Other product details
Dilution
1/2000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey® Blocker 1:1 in PBS + 0.1% Tween
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor 680 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000
Detection
Detection method
Odyssey® scanner
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 65 kDa Non-specific: 36 kDa
Positive control
HeLa Whole Cell Extract
Additional data
Additional Notes
Figure legend
WCE - HeLa Whole Cell Extract
WCE - HeLa Whole Cell Extract
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 29 2012