Key features and details
- Rabbit polyclonal to FANCC
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.878% Sodium chloride
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab5065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).|
功能DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Upon IFNG induction, may facilitate STAT1 activation by recruiting STAT1 to IFNGR1.
疾病相关Defects in FANCC are the cause of Fanconi anemia complementation group C (FANCC) [MIM:227645]. A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
发展阶段Expression increases during S phase, is maximal at the G2/M transition, and declines during M phase (at protein level).
细胞定位Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
- Information by UniProt
- bA80I15.1 antibody
- FA 3 antibody
- FA3 antibody
Anti-FANCC antibody (ab5065) at 1/1500 dilution + HeLa whole cell lysate at 35 µg
IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 63 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa. We are unsure as to the identity of these extra bands.
Primary antibody incubated with PBS supplemented with 1% normal goat serum and 0.1% milk.
ICC/IF image of ab5065 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5065, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab5065 被引用在 2 文献中.
- Lu WT et al. The Fanconi anemia pathway is downregulated upon macrophage differentiation through two distinct mechanisms. Cell Cycle 10: (2011). WB ; Human . PubMed: 21926477
- Palagyi A et al. Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents. Mol Cancer 9:127 (2010). WB ; Human . PubMed: 20509860