重组Anti-FANCA/FAA抗体[EPR16519] (ab201457)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16519] to FANCA/FAA
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FANCA/FAA抗体[EPR16519]
参阅全部 FANCA/FAA 一抗 -
描述
兔单克隆抗体[EPR16519] to FANCA/FAA -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, HEK-293, A431, A549, HAP1 and Jurkat whole cell lysates; Human colon lysate. IP: HeLa whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR16519 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab201457于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
1/30.
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WB |
1/1000. Detects a band of approximately 163 kDa (predicted molecular weight: 163 kDa).
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说明 |
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IP
1/30. |
WB
1/1000. Detects a band of approximately 163 kDa (predicted molecular weight: 163 kDa). |
靶标
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功能
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. -
疾病相关
Defects in FANCA are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H. -
细胞定位
Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic. - Information by UniProt
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数据库链接
- Entrez Gene: 2175 Human
- Omim: 607139 Human
- SwissProt: O15360 Human
- Unigene: 290154 Human
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别名
- FA 1 antibody
- FA antibody
- FA H antibody
see all
图片
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All lanes : Anti-FANCA/FAA antibody [EPR16519] (ab201457) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : FANCA knockout A549 cell lysate
Lane 3 : Wild-type HAP1 cell lysate
Lane 4 : FANCA knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 163 kDa
Observed band size: 163 kDaWestern blot: Anti-FANCA antibody [EPR16519] (ab201457) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab201457 was shown to bind specifically to FANCA. A band was observed at 163 kDa in wild-type A549 cell lysates with no signal observed at this size in FANCA knockout cell line. To generate this image, wild-type and FANCA knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: FANCA/FAA beta knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
Lanes 4, 8 and 12: HEK293 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target – ab201457 observed at 163 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signalab201457 was shown to specifically react with FANCA/FAA beta when FANCA/FAA beta knockout samples were used. Wild-type and FANCA/FAA beta knockout samples were subjected to SDS-PAGE. ab201457 and ab8245 (loading control to GAPDH) were diluted 1/10000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
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All lanes : Anti-FANCA/FAA antibody [EPR16519] (ab201457) at 1/10000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 4 : A431 (Human epidermoid carcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 163 kDa
Observed band size: 163 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-FANCA/FAA antibody [EPR16519] (ab201457) at 1/1000 dilution + Human colon lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 163 kDa
Observed band size: 163 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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FANCA/FAA was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab201457 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201457 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input). Lane 2: ab201457 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201457 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 30 seconds.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab201457 被引用在 3 文献中.
- Kalev P et al. MAT2A Inhibition Blocks the Growth of MTAP-Deleted Cancer Cells by Reducing PRMT5-Dependent mRNA Splicing and Inducing DNA Damage. Cancer Cell 39:209-224.e11 (2021). PubMed: 33450196
- Hambarde S et al. EXO5-DNA structure and BLM interactions direct DNA resection critical for ATR-dependent replication restart. Mol Cell 81:2989-3006.e9 (2021). PubMed: 34197737
- Zhong J et al. Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress. Int J Biol Sci 13:923-934 (2017). PubMed: 28808424