Anti-FANCA/FAA抗体(ab5063)
Key features and details
- Rabbit polyclonal to FANCA/FAA
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-FANCA/FAA抗体
参阅全部 FANCA/FAA 一抗 -
描述
兔多克隆抗体to FANCA/FAA -
宿主
Rabbit -
特异性
Detects a band at 130kDa in HeLa cell lysate that we believe corresponds to FANCA/FAA.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human FANCA/FAA aa 995-1009 conjugated to keyhole limpet haemocyanin.
Sequence:SRSYDHSENSDLVFG-C
Database link: O15360 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
1/500 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 163 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 163 kDa). |
靶标
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功能
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. -
疾病相关
Defects in FANCA are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H. -
细胞定位
Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic. - Information by UniProt
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数据库链接
- Entrez Gene: 2175 Human
- Omim: 607139 Human
- SwissProt: O15360 Human
- Unigene: 290154 Human
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别名
- FA 1 antibody
- FA antibody
- FA H antibody
see all
图片
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Western blot - Anti-FANCA/FAA antibody (ab5063)Anti-FANCA/FAA antibody (ab5063) at 1/1500 dilution + HeLa whole cell lysate at 35 µg
Secondary
IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 163 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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Datasheet download
文献 (10)
ab5063 被引用在 10 文献中.
- Román-Rodríguez FJ et al. NHEJ-Mediated Repair of CRISPR-Cas9-Induced DNA Breaks Efficiently Corrects Mutations in HSPCs from Patients with Fanconi Anemia. Cell Stem Cell 25:607-621.e7 (2019). PubMed: 31543367
- Abdul-Sater Z et al. FANCA safeguards interphase and mitosis during hematopoiesis in vivo. Exp Hematol 43:1031-1046.e12 (2015). IF . PubMed: 26366677
- Vanuytsel K et al. FANCA knockout in human embryonic stem cells causes a severe growth disadvantage. Stem Cell Res 13:240-50 (2014). WB . PubMed: 25108529
- Rio P et al. Targeted gene therapy and cell reprogramming in Fanconi anemia. EMBO Mol Med 6:835-48 (2014). WB ; Human . PubMed: 24859981
- Shen C et al. Regulation of FANCD2 by the mTOR pathway contributes to the resistance of cancer cells to DNA double-strand breaks. Cancer Res 73:3393-401 (2013). PubMed: 23633493
- Meier D & Schindler D Fanconi anemia core complex gene promoters harbor conserved transcription regulatory elements. PLoS One 6:e22911 (2011). WB . PubMed: 21826217
- Lu WT et al. The Fanconi anemia pathway is downregulated upon macrophage differentiation through two distinct mechanisms. Cell Cycle 10: (2011). WB ; Human . PubMed: 21926477
- Tulpule A et al. Knockdown of Fanconi anemia genes in human embryonic stem cells reveals early developmental defects in the hematopoietic lineage. Blood 115:3453-62 (2010). WB ; Human . PubMed: 20089964
- Mattera L et al. The E1A-associated p400 protein modulates cell fate decisions by the regulation of ROS homeostasis. PLoS Genet 6:e1000983 (2010). WB ; Human . PubMed: 20548951
- Wilson JB et al. FANCG promotes formation of a newly identified protein complex containing BRCA2, FANCD2 and XRCC3. Oncogene 27:3641-52 (2008). PubMed: 18212739
