Key features and details
- Rabbit monoclonal [EP841Y] to FAK (phospho Y861)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
产品名称Anti-FAK (phospho Y861)抗体[EP841Y]
参阅全部 FAK 一抗
描述兔单克隆抗体[EP841Y] to FAK (phospho Y861)
经测试应用适用于: WBmore details
不适用于: Flow Cyt,ICC,IHC-P or IP
种属反应性与反应: Mouse, Rat, Human
Synthetic peptide within Human FAK (phospho Y861). The exact sequence is proprietary.
- Hela cell lysates treated with pervanadate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab81293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 119 kDa.|
功能Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
组织特异性Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
结构域The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
翻译后修饰Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
细胞定位Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
- Information by UniProt
- FADK 1 antibody
- FADK antibody
- FAK related non kinase polypeptide antibody
All lanes : Anti-FAK (phospho Y861) antibody [EP841Y] (ab81293) at 1/50000 dilution
Lane 1 : HeLa cell lysates treated with pervanadate
Lane 2 : HeLa cell lysates, untreated
All lanes : HRP goat ant-rabbit IgG (H+L) at 1/2000 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
ab81293 被引用在 4 文献中.
- Zhang YL et al. Integrin a9 Suppresses Hepatocellular Carcinoma Metastasis by Rho GTPase Signaling. J Immunol Res 2018:4602570 (2018). PubMed: 29951557
- Luo X et al. Bioinformatics analysis of microarray profiling identifies the mechanism of focal adhesion kinase signalling pathway in proliferation and apoptosis of breast cancer cells modulated by green tea polyphenol epigallocatechin 3-gallate. J Pharm Pharmacol 70:1606-1618 (2018). PubMed: 30187481
- Xu Z et al. Integrin ß1 is a critical effector in promoting metastasis and chemo-resistance of esophageal squamous cell carcinoma. Am J Cancer Res 7:531-542 (2017). PubMed: 28401009
- Wu J et al. AURKA promotes cell migration and invasion of head and neck squamous cell carcinoma through regulation of the AURKA/Akt/FAK signaling pathway. Oncol Lett 11:1889-1894 (2016). PubMed: 26998095