Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231 human breast cancer cells)
Loading amount
25 µg
Specification
MDA-MB-231 human breast cancer cells
Treatment
either untreated or treated with 2.5 or 5uM src inhibitor AZD0530
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 125 kDa
Positive control
left- untreated MDA-MB-231 cells
Negative control
MDA-MB-231 cells treated for 1hr with 2.5uM (middle) and 5uM (right) AZD0530 src inhibitor
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提交于 Sep 17 2010